| Texto completo | |
| Autor(es): |
Liberato, Marcelo Vizona
;
Generoso, Wesley Cardoso
;
Malago, Wilson, Jr.
;
Henrique-Silva, Flavio
;
Polikarpov, Igor
Número total de Autores: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS; v. 68, p. 4-pg., 2012-03-01. |
| Resumo | |
Endoglucanases are enzymes that hydrolyze cellulose and are important components of the cellulolytic complex. In contrast to other members of the complex, they cleave internal beta-1,4-glycosidic bonds in the cellulose polymer, allowing cellulose to be used as an energy source. Since biomass is an important renewable source of energy, the structural and functional characterization of these enzymes is of interest. In this study, endoglucanase III from Trichoderma harzianum was produced in Pichia pastoris and purified. Crystals belonging to the orthorhombic space group P212121, with unit-cell parameters a = 47.54, b = 55.57, c = 157.3 angstrom, were obtained by the sitting-drop vapour-diffusion method and an X-ray diffraction data set was collected to 2.07 angstrom resolution. (AU) | |
| Processo FAPESP: | 09/52840-7 - Centro de Processos Biológicos e Industriais para Biocombustíveis - CeProBIO |
| Beneficiário: | Igor Polikarpov |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOEN - Temático |
| Processo FAPESP: | 08/56255-9 - Structure and function of enzymes and auxiliary proteins from Trichoderma, active in cell-wall hydrolysis |
| Beneficiário: | Igor Polikarpov |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOEN - Temático |
| Processo FAPESP: | 09/14333-6 - Caracterização estrutural dos complexos entre os receptores ativadores da proliferação de peroxissomos (PPARs) dos tipos alfa e gama e seus agonistas |
| Beneficiário: | Jademilson Celestino dos Santos |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |