SPEM: A state-of-the-art instrument for high resolution molecular imaging of small animal organs

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dos Reis, Marilia Alves ; Mejia, Jorge ; Batista, Ilza Rosa ; Felisa Figols de Barboza, Marycel Rosa ; Nogueira, Solange Amorim ; Wagner, Jairo ; Cabral, Francisco Romero ; Victorio Mendonca Davoglio, Petrick Marcellus ; Abilio, Vanessa Costhek ; Fu, Geng ; Li, Nan ; Meng, Ling Jian ; Shih, Ming Chi ; Chen, Chin-Tu ; Junior, Edson Amaro ; Bressan, Rodrigo Affonseca

• Tipo de documento: Artigo Científico
• Fonte: Einstein (São Paulo); v. 10, n. 2, p. 7-pg., 2012-04-01.

Resumo

Objective: To describe the Single Photon Emission Microscope (SPEM), a state-of-the-art instrument for small animal SPECT imaging, and characterize its performance presenting typical images of different animal organs. Methods: SPEM consists of two independent imaging devices based on high resolution scintillators, high sensitivity and resolution Electron-Multiplying CCDs and multi-pinhole collimators. During image acquisition, the mouse is placed in a rotational vertical holder between the imaging devices. Subsequently, an appropriate software tool based on the Maximum Likelihood algorithm iteratively produces the volumetric image. Radiopharmaceuticals for imaging kidneys, heart, thyroid and brain were used. The mice were injected with 74 to 148 MBq/0,3mL and scanned for 40 to 80 minutes, 30 to 60 minutes afterwards. During this procedure, the animals remained under ketamine/xilazine anesthesia. Results: SPEM images of different mouse organs are presented, attesting the imaging capabilities of the instrument. Conclusion: SPEM is an innovative technology for small animal SPECT imaging providing high resolution images with appropriate sensitivity for pre-clinical research. Its use with appropriate radiotracers will allow translational investigation of several animal models of human diseases, their pharmacological treatment and the development of potential new therapeutic agents. (AU)

• Processo FAPESP: 08/08902-5 - Avaliação de radiotraçadores para Neuroimagem Molecular de receptores dopaminérgicos e serotoninérgicos em modelos animais in vivo através de SPECT comparando técnicas de binding in vitro e auto-radiografia ex vivo.
• Beneficiário: Marilia Alves dos Reis
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 06/61333-3 - Núcleo de pesquisa em neuroimagem pré-clínica (centro de neuroimagem experimental)
• Beneficiário: Luiz Augusto Franco de Andrade
• Modalidade de apoio: Auxílio à Pesquisa - Temático