| Texto completo | |
| Autor(es): |
Giannotti, Karina Cristina
;
Leiguez, Elbio
;
Moreira, Vanessa
;
Nascimento, Neide Galvao
;
Lomonte, Bruno
;
Maria Gutierrez, Jose
;
de Melo, Robson Lopes
;
Teixeira, Catarina
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BIOMED RESEARCH INTERNATIONAL; v. 2013, p. 14-pg., 2013-01-01. |
| Resumo | |
MT-II, a Lys49PLA(2) homologue devoid of catalytic activity from B. asper venom, stimulates inflammatory events in macrophages. e investigated the ability of MT-II to induce formation of lipid droplets (LDs), key elements of inflammatory responses, in isolated macrophages and participation of protein kinases and intracellular PLA(2)s in this effect. Influence of MT-II on PLI 2 recruitment and expression was assessed, and the effects of some synthetic peptides on LD formation were further evaluated. At noncytotoxic concentrations, MT-II directly activated macrophages to form LDs.. is effect was reproduced by a synthetic peptide corresponding to the C-terminal sequence 115-129 of MT-II, evidencing the critical role of C-terminus for MT-II-induced effect. Moreover, MT-II induced expression and recruitment of PLIN2. Pharmacological interventions with specific inhibitors showed that PKC, PI3K, ERK1/2, and iPLA(2), but not P38(MAPK) or cPLA(2), signaling pathways are involved in LD formation induced by MT-II. This sPLA(2) homologue also induced synthesis of PGE(2) that colocalized to LDs. In conclusion, MT-II is able to induce formation of LDs committed to PGE(2) formation in a process dependent on C-terminal loop engagement and regulated by distinct protein kinases and iPLA(2). LDs may constitute an important inflammatory mechanism triggered by MT-II in macrophages. (AU) | |
| Processo FAPESP: | 07/03337-5 - Efeito de duas fosfolipases A2, isoladas do veneno de serpente, sobre vias de ativação do NF-kapa b relacionadas à expressão de COX-2 e de PGE sintase m-1: envolvimento do receptor de manose de macrófagos nesses eventos |
| Beneficiário: | Vanessa Moreira |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 10/06345-1 - Estudo dos fatores envolvidos na formação de corpúsculos lipídicos, induzidos por uma Fosfolipase A2, isolada do veneno de serpente: síntese e metabolismo de lipídios |
| Beneficiário: | Elbio Leiguez Junior |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 11/21341-5 - Estudo de mecanismos envolvidos na formação de corpúsculos lipídicos induzidos por uma fosfolipase A2 isolada do veneno da serpente Bothrops asper em macrófagos isolados: participação de fatores da homeostasia lipídica |
| Beneficiário: | Catarina de Fatima Pereira Teixeira |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |