| Texto completo | |
| Autor(es): |
Zanelato, A. I.
;
Shiota, V. M.
;
Gomes, E.
;
da Silva, R.
;
Thomeo, J. C.
Número total de Autores: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Brazilian Journal of Microbiology; v. 43, n. 4, p. 9-pg., 2012-10-01. |
| Resumo | |
This work is aimed to produce endoglucanase through solid state fermentation in a packed bed bioreactor with the use of the fungus Myceliophtora sp. I-1D3b using a mixture of wheat bran (WB) and sugar cane bagasse (SCB) as culture medium. Preliminary tests were performed in polypropylene plastic bags, controlling the variables temperature (40, 45, and 50 degrees C), initial moisture content (75, 80, and 85%, w.b.), and weight proportion SCB/WB (1: 1, 7: 3, and 9: 1). The highest enzyme activities in plastic bags were obtained using the substrate proportion of 7: 3, 50 degrees C temperature, and 80% initial moisture content (878 U/grams of dry solid). High activities of filter-paper cellulase and xylanase were also obtained in plastic bags and some results are reported. For the packed bed experiments, the temperature (45 and 50 degrees C) and the air flow rate (80, 100 and 120L/h) were the controlled variables. Activity of endoglucanase was similar to plastic bag tests. A longitudinal gradient of moisture content, was observed increasing from the bottom to the top of the reactor, even though the longitudinal enzyme activity profile was flat for almost the whole bed. Air flow rate did not affect enzyme activity, while experiments carried out at 50 degrees C showed higher enzyme activities. The maximum temperature peak observed was at about 6 degrees C above the process temperature. (AU) | |
| Processo FAPESP: | 10/12624-0 - Aplicação de métodos físico-químicos e enzimáticos na sacarificação do bagaço de cana: estudos de microrganismos, processos fermentativos relacionados e métodos de hidrólise |
| Beneficiário: | Eleni Gomes |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 08/52811-4 - Producao de enzimas celuloliticas por fermentacao em estado solido em biorretor de leito fixo. |
| Beneficiário: | Alex Izuka Zanelato |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |