Crystallization and preliminary X-ray diffraction analysis of a new xyloglucanase from Xanthomonas campestris pv. campestris

Autor(es)

de Araujo, Evandro Ares ; Tomazini, Atilio, Jr. ; Seiki Kadowaki, Marco Antonio ; Murakami, Mario Tyago ; Polikarpov, Igor

• Tipo de documento: Artigo Científico
• Fonte: ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS; v. 69, p. 3-pg., 2013-06-01.

Resumo

Xyloglucanases (Xghs) are important enzymes involved in xyloglucan modification and degradation. Xanthomonas campestris pv. campestris (Xcc) is a phytopathogenic bacterium which produces a large number of glycosyl hydrolases (GH), but has only one family 74 GH (Xcc-Xgh). This enzyme was overexpressed in Escherichia coli, purified and crystallized. Diffraction data sets were collected for the native enzyme and its complex with glucose to maximum resolutions of 2.0 and 2.1 angstrom, respectively. The data were indexed in a hexagonal crystal system with unit-cell parameters a = b = 153.4, c = 84.9 angstrom. As indicated by molecular-replacement solution, the crystals belonged to space group P6(1). (AU)

• Processo FAPESP: 09/05349-6 - Expressão e purificação da celobiohidrolase I do fungo filamentoso Trichoderma harzianum
• Beneficiário: Bruno Luan Soares Paula de Mello
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 10/52362-5 - Targeted analysis of microbial lignocellulolytic secretomes: a new approach to enzyme discovery
• Beneficiário: Igor Polikarpov
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/56255-9 - Structure and function of enzymes and auxiliary proteins from Trichoderma, active in cell-wall hydrolysis
• Beneficiário: Igor Polikarpov
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 07/08706-9 - ESTUDOS MOLECULARES E ESTRUTURAIS DE ENZIMAS DO COMPLEXO CELULOLÍTICO DO FUNGO Trichoderma harzianum COM POTENCIAL NA TRANSFORMAÇÃO ENZIMÁTICA DA BIOMASSA
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