Detection of Bovine Leukemia Virus in Brains of Cattle with a Neurological Syndrome: Pathological and Molecular Studies

Autor(es)

Ramos D'Angelino, Rubens Henrique ; Pituco, Edviges Maristela ; Cassaro Villalobos, Eliana Monteforte ; Harakava, Ricardo ; Gregori, Fabio ; Del Fava, Claudia

• Tipo de documento: Artigo Científico
• Fonte: BIOMED RESEARCH INTERNATIONAL; v. 2013, p. 6-pg., 2013-01-01.

Resumo

Bovine leukemia virus (BLV) was investigated in the central nervous system (CNS) of cattle with neurological syndrome. A total of 269 CNS samples were submitted to nested-PCR (BLV env gene gp51), and the viral genotypes were identified. The nested-PCR was positive in 4.8% (13/269) CNS samples, with 2.7% (2/74) presenting at histological examination lesions of nonpurulent meningoencephalitis (NPME), whereas 5.6% (11/195) not presenting NPME (P > 0.05). No samples presented lymphosarcoma. The PCR products (437 bp) were sequenced and submitted to phylogenetic analysis by neighbor-joining and maximum composite likelihood methods, and genotypes 1, 5, and 6 were detected, corroborating other South American studies. The genotype 6 barely described in Brazil and Argentina was more frequently detected in this study. The identity matrices showed maximum similarity (100%) among some samples of this study and one from Argentina (FJ808582), recovered from GenBank. There was no association among the genotypes and NPME lesions. (AU)

• Processo FAPESP: 10/04692-6 - Gestão sanitária das encefalites em bovinos: vírus da leucemia bovina como agente causal
• Beneficiário: Rubens Henrique Ramos D'Angelino
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 11/06513-4 - Gestão sanitária das encefalites em bovinos: vírus da leucemia bovina como agente causal
• Beneficiário: Claudia Del Fava
• Modalidade de apoio: Auxílio à Pesquisa - Regular