New trends in LC protein ligand screening

Autor(es)

de Moraes, Marcela C. ; Vanzolini, Kenia L. ; Cardoso, Carmen L. ; Cass, Quezia B.

• Tipo de documento: Artigo Científico
• Fonte: Journal of Pharmaceutical and Biomedical Analysis; v. 87, p. 12-pg., 2014-01-18.

Resumo

The diversity of small molecules available to produce truly innovative drugs associated with the wealth of known biological targets calls for key strategies in protein ligand screening. This review encompasses the recently developed bioaffinity-based strategies. A critical view of the use of zonal, frontal, and nonlinear chromatography with immobilized proteins is given. The association of these elution modes with the ligand fishing method, which uses nanomagnetics particles, is also addressed. A series of applications and how these new screening strategies can be used to determine the function, affinity, and activity parameters of proteins is discussed. (C) 2013 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 08/04371-5 - Desenvolvimento e aplicação de colunas enzimáticas na triagem de inibidores seletivos e no monitoramento do trabalho fitoquímico de produtos naturais
• Beneficiário: Carmen Lúcia Cardoso
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores