Chitosan microlayer on the photografting modified surface of PLA, PCL and PLA/PCL bioextruder scaffolds

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Loaiza, M. ; Rezende, R. A. ; da Silva, J. V. L. ; Bartolo, P. J. ; Sabino, M. A. ; Bartolo, PJD ; DeLemos, ACS ; Pereira, AMH ; Mateus, AJD ; Ramos, C ; DosSantos, C ; Oliveira, D ; Pinto, E ; Craveiro, F ; Bartolo, HMCDTG ; Almeia, HD ; Sousa, I ; Matias, JM ; Durao, L ; Gaspar, M ; Alves, NMF ; Carreira, P ; Ferreira, T ; Marques, T

• Tipo de documento: Artigo Científico
• Fonte: HIGH VALUE MANUFACTURING: ADVANCED RESEARCH IN VIRTUAL AND RAPID PROTOTYPING; v. N/A, p. 5-pg., 2014-01-01.

Resumo

Polylactic (PLA), Polycaprolactone (PCL) and blend of Polylactid/Polycaprolactone (PLA/PCL) scaffolds constructs composed by layers of microsized filaments (0/90. lay-down pattern), with a diameter of around 400 m and interfilament distance of around 200 m and 10 layers, were produced using a melt extrusion-based additive manufacturing technique. The range composition of these blends used for scaffolding was PLA/PCL [% w/w]= 100/0, 90/10, 80/20, 0/100; and also a graft PLA with maleic anhydride was incorporate as compatibilizer to blend PLA/PLA-g-AM/PCL= 80/10/10 [% w/w]. All polymer blends were prepared using solvent casting technique to avoid thermal degradation during blending. These blends were characterized using Differential Scanning Calorimetric (DSC) to know about compatibility and thermal properties to construct the scaffolds by melt extrusion-additive manufacturing technique. In general, one of the limitations to the use of PLA, PLC or PLA/PCL polymer blends as an ideal biomaterial with great biocompatibility is their high hydrofobicity. To improve the hydrofilicity and biocompatibility, after the scaffolds built, a chitosan microlayer was grafted onto scaffolds surface through the combination of photooxidation in hydrogen peroxide/UV exposition. Photo-oxidation of these surfaces modification was evaluated using Attenuated Total Reflectance-Fourier Transformed Infrared (ATR-FTIR). After the activation of the scaffolds surface, they were immersed into a chitosan solution, and finally morphologically evaluated using scanning electron microscopy. Results show that the surface modificationwas more effective in all blends with PLA phase, and the microlayer of chitosan showed good adhesion to the same cases, allowing infer their biocompatible character. (AU)

• Processo FAPESP: 12/19302-4 - Construções poliméricas em dupla escala como scaffolds para a engenharia tecidual usando poliéster biodegradável e biopolímeros
• Beneficiário: Jorge Vicente Lopes da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisador Visitante - Internacional
• Processo FAPESP: 10/20763-0 - Engenharia tecidual por meio de simulação dos esferóides como blocos de construção na biofabricação
• Beneficiário: Rodrigo Alvarenga Rezende
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado