Chitosan-tripolyphosphate nanoparticles as Arrabidaea chica standardized extract carrier: synthesis, characterization, biocompatibility, and antiulcerogenic activity

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Servat-Medina, Leila ; Gonzalez-Gomez, Alvaro ; Reyes-Ortega, Felisa ; Oliveira Sousa, Ilza Maria ; Almeida Queiroz, Nubia de Cassia ; Wiziack Zago, Patricia Maria ; Jorge, Michelle Pedrosa ; Monteiro, Karin Maia ; de Carvalho, Joao Ernesto ; San Roman, Julio ; Foglio, Mary Ann

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF NANOMEDICINE; v. 10, p. 13-pg., 2015-01-01.

Resumo

Natural products using plants have received considerable attention because of their potential to treat various diseases. Arrabidaea chica (Humb. & Bonpl.) B. Verlot is a native tropical American vine with healing properties employed in folk medicine for wound healing, inflammation, and gastrointestinal colic. Applying nanotechnology to plant extracts has revealed an advantageous strategy for herbal drugs considering the numerous features that nanostructured systems offer, including solubility, bioavailability, and pharmacological activity enhancement. The present study reports the preparation and characterization of chitosan-sodium tripolyphosphate nanoparticles (NPs) charged with A. chica standardized extract (AcE). Particle size and zeta potential were measured using a Zetasizer Nano ZS. The NP morphological characteristics were observed using scanning electron microscopy. Our studies indicated that the chitosan/sodium tripolyphosphate mass ratio of 5 and volume ratio of 10 were found to be the best condition to achieve the lowest NP sizes, with an average hydrodynamic diameter of 150 +/- 13 nm and a zeta potential of +45 +/- 2 mV. Particle size decreased with AcE addition (60 +/- 10.2 nm), suggesting an interaction between the extract's composition and polymers. The NP biocompatibility was evaluated using human skin fibroblasts. AcE-NP demonstrated capability of maintaining cell viability at the lowest concentrations tested, stimulating cell proliferation at higher concentrations. Antiulcerogenic activity of AcE-NP was also evaluated with an acute gastric ulcer experimental model induced by ethanol and indomethacin. NPs loaded with A. chica extract reduced the ulcerative lesion index using lower doses compared with the free extract, suggesting that extract encapsulation in chitosan NPs allowed for a dose reduction for a gastroprotective effect. The AcE encapsulation offers an approach for further application of the A. chica extract that could be considered a potential candidate for ulcer-healing pharmaceutical systems. (AU)

• Processo FAPESP: 09/51020-6 - Atividade cicatrizante de microencapsulados de extrato bruto etanolico obtidos de arrabidaea chica verlot.
• Beneficiário: Michelle Pedroza Jorge
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/16484-1 - Arrabidaea chica Verlot: Formulações de liberação sustentada para aplicação em úlceras de mucosa e pele.
• Beneficiário: Leila Servat Medina
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/07781-0 - Arrabidaea chica Verlot: avaliação in vitro de toxicidade, atividades antimicrobiana e citoprotetora do extrato bruto livre padronizado combinado ao ácido zoledrônico, e estudos de microencapsulação
• Beneficiário: Patrícia Maria Wiziack Zago
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/09127-8 - Arrabidaea chica Verlot: micro e nanoencapsulação de extratos brutos padronizados em carajiruna e sua influência na cicatrização de lesões de pele e mucosa
• Beneficiário: Mary Ann Foglio
• Modalidade de apoio: Auxílio à Pesquisa - Regular