Negative Energy Balance Induced by Paradoxical Sleep Deprivation Causes Multicompartmental Changes in Adipose Tissue and Skeletal Muscle

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Monico-Neto, Marcos ; de Campos Giampa, Sara Quaglia ; Lee, Kil Sun ; de Melo, Camila Maria ; Souza, Helton de Sa ; Dattilo, Murilo ; Minali, Paulo Alexandre ; Santos Prado, Pedro Henrique ; Tufik, Sergio ; de Mello, Marco Tulio ; Moreira Antunes, Hanna Karen

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF ENDOCRINOLOGY; v. 2015, p. 6-pg., 2015-01-01.

Resumo

Objective. Describe multicompartmental changes in the fat and various muscle fiber types, as well as the hormonal profile and metabolic rate induced by SD in rats. Methods. Twenty adult male Wistar rats were equally distributed into two groups: experimental group (EG) and control group (CG). The EG was submitted to SD for 96 h. Blood levels of corticosterone (CORT), total testosterone (TESTO), insulin like growth factor-1 (IGF-1), and thyroid hormones (T3 and T4) were used to assess the catabolic environment. Muscle trophism was measured using a cross-sectional area of various muscles (glycolytic, mixed, and oxidative), and lipolysis was inferred by the weight of fat depots from various locations, such as subcutaneous, retroperitoneal, and epididymal. The metabolic rate was measured using oxygen consumption (VO2) measurement. Results. SD increased CORT levels and decreased TESTO, IGF-1, and T4. All fat depots were reduced in weight after SD. Glycolytic and mixed muscles showed atrophy, whereas atrophy was not observed in oxidative muscle. Conclusion. Our data suggest that glycolytic muscle fibers are more sensitive to atrophy than oxidative fibers during SD and that fat depots are reduced regardless of their location. (AU)

• Processo FAPESP: 13/00152-5 - Efeito do exercício físico resistido no músculo esquelético e cardíaco de ratos Wistar submetidos a privação de sono paradoxal
• Beneficiário: Hanna Karen Moreira Antunes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 98/14303-3 - Center for Sleep Studies
• Beneficiário: Sergio Tufik
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 11/15962-7 - Efeitos do exercício físico resistido nas vias tróficas e atróficas da musculatura esquelética de ratos submetidos à privação de sono paradoxal
• Beneficiário: Marcos Mônico Neto
• Modalidade de apoio: Bolsas no Brasil - Mestrado