Comparative Study of the Accuracy of Different Techniques for the Laboratory Diagnosis of Schistosomiasis Mansoni in Areas of Low Endemicity in Barra Mansa City, Rio de Janeiro State, Brazil

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Carvalho Espirito-Santo, Maria Cristina ; Alvarado-Mora, Monica Viviana ; Silva Pinto, Pedro Luiz ; Arroyo Sanchez, Maria Carmen ; Dias-Neto, Emmanuel ; Pagliusi Castilho, Vera Lucia ; do Nascimento Goncalves, Elenice Messias ; Chieffi, Pedro Paulo ; de Albuquerque Luna, Expedito Jose ; Rebello Pinho, Joao Renato ; Carrilho, Flair Jose ; Borges Gryschek, Ronaldo Cesar

• Tipo de documento: Artigo Científico
• Fonte: BIOMED RESEARCH INTERNATIONAL; v. 2015, p. 16-pg., 2015-01-01.

Resumo

Schistosomiasis constitutes a major public health problem, with an estimated 200 million people infected worldwide. Many areas of Brazil show low endemicity of schistosomiasis, and the current standard parasitological techniques are not sufficiently sensitive to detect the low-level helminth infections common in areas of low endemicity (ALEs). This study compared the Kato-Katz (KK); Hoffman, Pons, and Janer (HH); enzyme-linked immunosorbent assay-(ELISA-) IgG and ELISA-IgM; indirect immunofluorescence technique (IFT-IgM); and qPCR techniques for schistosomiasis detection in serum and fecal samples, using the circumoval precipitin test (COPT) as reference. An epidemiological survey was conducted in a randomized sample of residents from five neighborhoods of Barra Mansa, RJ, with 610 fecal and 612 serum samples. ELISA-IgM(21.4%) showed the highest positivity and HH and KK techniques were the least sensitive (0.8%). All techniques except qPCR-serum showed high accuracy (82-95.5%), differed significantly from COPT in positivity (P < 0.05), and showed poor agreement with COPT. Medium agreement was seen with ELISA-IgG (Kappa = 0.377) and IFA (Kappa = 0.347). Parasitological techniques showed much lower positivity rates than those by other techniques. We suggest the possibility of using a combination of laboratory tools for the diagnosis of schistosomiasis in ALEs. (AU)

• Processo FAPESP: 08/50461-6 - Estudo da variabilidade genotípica dos vírus da Hepatite B e Hepatite C na Colômbia
• Beneficiário: Monica Viviana Alvarado Mora
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 10/52615-0 - Estudo comparativo de metodos diagnosticos da esquistossomose mansoni em areas de baixa transmissao e prevalencia.
• Beneficiário: Ronaldo Cesar Borges Cryschek
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/53457-7 - Estudo da variabilidade genotípica dos vírus das Hepatites B e C na Colômbia
• Beneficiário: João Renato Rebello Pinho
• Modalidade de apoio: Auxílio à Pesquisa - Regular