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Long-Standing Motor and Sensory Recovery following Acute Fibrin Sealant Based Neonatal Sciatic Nerve Repair

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Autor(es):
Biscola, Natalia Perussi ; Cartarozzi, Luciana Politti ; Ferreira Junior, Rui Seabra ; Barraviera, Benedito ; Rodrigues de Oliveira, Alexandre Leite
Número total de Autores: 5
Tipo de documento: Artigo Científico
Fonte: NEURAL PLASTICITY; v. 2016, p. 19-pg., 2016-01-01.
Resumo

Brachial plexus lesion results in loss of motor and sensory function, being more harmful in the neonate. Therefore, this study evaluated neuroprotection and regeneration after neonatal peripheral nerve coaptation with fibrin sealant. Thus, P2 neonatal Lewis rats were divided into three groups: AX: sciatic nerve axotomy (SNA) without treatment; AX+FS: SNA followed by end-to-end coaptation with fibrin sealant derived from snake venom; AX+CFS: SNA followed by end-to-end coaptation with commercial fibrin sealant. Results were analyzed 4, 8, and 12 weeks after lesion. Astrogliosis, microglial reaction, and synapse preservation were evaluated by immunohistochemistry. Neuronal survival, axonal regeneration, and ultrastructural changes at ventral spinal cord were also investigated. Sensory-motor recovery was behaviorally studied. Coaptation preserved synaptic covering on lesioned motoneurons and led to neuronal survival. Reactive gliosis and microglial reaction decreased in the same groups (AX+FS, AX+CFS) at 4 weeks. Regarding axonal regeneration, coaptation allowed recovery of greater number of myelinated fibers, with improved morphometric parameters. Preservation of inhibitory synaptic terminals was accompanied by significant improvement in the motor as well as in the nociceptive recovery. Overall, the present data suggest that acute repair of neonatal peripheral nerves with fibrin sealant results in neuroprotection and regeneration of motor and sensory axons. (AU)

Processo FAPESP: 14/06892-3 - Utilização de células tronco mesenquimais na interface do sistema nervoso central e periférico: reparo de lesões proximais
Beneficiário:Alexandre Leite Rodrigues de Oliveira
Modalidade de apoio: Auxílio à Pesquisa - Temático
Processo FAPESP: 09/53846-9 - EMU: aquisição de cromatógrafo líquido de alta eficiência (CLAE) preparativo para purificação e isolamento de serinoproteinases para produção do selante de fibrina
Beneficiário:Benedito Barraviera
Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
Processo FAPESP: 12/08101-8 - Imageamento in vivo da regeneração nervosa após reimplante de raízes motoras com selante de fibrina fluo-marcado associado com células tronco mesenquimais
Beneficiário:Rui Seabra Ferreira Junior
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 11/23236-4 - Toxinas animais nativas e recombinantes: análise funcional, estrutural e molecular
Beneficiário:Suely Vilela
Modalidade de apoio: Auxílio à Pesquisa - Temático
Processo FAPESP: 11/23377-7 - Efeitos neuroprotetor e regenerativo do selante de fibrina derivado da peçonha de serpente combinado com células tronco mononucleares após neurorrafia do nervo isquiático de ratos neonatos
Beneficiário:Natália Perussi Biscola
Modalidade de apoio: Bolsas no Brasil - Doutorado Direto