Evidence of heterozygosity and recombinant alleles in single cysts of Giardia duodenalis

Autor(es)

Aguiar, Juliana Martins ; Silva, Sheila Oliveira ; dos Santos, Valdir Azevedo ; Taniwaki, Sueli Akemi ; Ferreira de Sousa Oliveira, Tricia Maria ; Ferreira, Helena Lage ; Keid, Lara Borges ; Gregori, Fabio ; Soares, Rodrigo Martins

• Tipo de documento: Artigo Científico
• Fonte: REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA; v. 25, n. 2, p. 9-pg., 2016-04-01.

Resumo

Giardia duodenalis is divided into eight assemblages (named A to H). Isolates of assemblage A are divided into four sub-assemblages (AI, AII, AIII and AIV). While isolates of sub-assemblage AII are almost exclusively detected in human hosts, isolates of assemblage B are encountered in a multitude of animal hosts and humans. Here, we isolated single cysts of G. duodenalis from a human stool sample and found that one of them had overlaps of assemblage AII and B alleles and an unexpectedly high number of variants of the beta-giardin (Bg) and GLORF-C4 (OrfC4) alleles. In addition, one of the Bg alleles of that cyst had a fragment of sub-assemblage AII interspersed with fragments of assemblage B, thus indicating that this allele may be a recombinant between sequences A and B. Our results are unprecedented and put a check on the statement that different assemblages of G. duodenalis represent species with different host specificities. (AU)

• Processo FAPESP: 11/13472-2 - Genotipagem de Giardia duodenalis: detecção de infecções mistas e recombinações gênicas em amostras de origem humana
• Beneficiário: Juliana Martins Aguiar
• Modalidade de apoio: Bolsas no Brasil - Doutorado