Antimicrobial activity and substantivity of Uncaria tomentosa in infected root canal dentin

Autor(es)

Herrera, Daniel Rodrigo ; Durand-Ramirez, Jorge Enrique ; Falcao, Amanda ; Leal Nogueira da Silva, Emmanuel Joao ; dos Santos, Elizabete Brasil ; Figueiredo de Almeida Gomes, Brenda Paula

• Tipo de documento: Artigo Científico
• Fonte: BRAZILIAN ORAL RESEARCH; v. 30, n. 1, p. 5-pg., 2016-01-01.

Resumo

The aim of this study was to analyze the antimicrobial activity and substantivity of Uncaria tomentosa Willd DC (cat's claw, CC) in root dentin contaminated with Enterococcus faecalis. Forty-eight human premolars were contaminated with E. faecalis (ATCC 29212) and randomly divided into four groups according to the irrigant used during chemomechanical preparation (CMP): CC group: 2% CC gel; CHX group: 2% chlorhexidine digluconate gel (CHX); NaOCl group: 5.25% sodium hypochlorite (NaOCl); and SS group: sterile saline (SS). Microbiological samples were collected before (S1) and after (S2) CMP and after 7 days (S3). Colony-forming units (CFU/mL) at the different sampling times and comparisons among the groups were statistically analyzed by Wilcoxon and Kruskal-Wallis tests (p < 0.05). Significant bacterial reduction was achieved in all groups after CMP (p < 0.05). Results show no significant difference between S3 and S2 (p > 0.05) in the CC and CHX groups. Bacterial load was higher in S3 than in S2 samples (p < 0.05) in the NaOCl and SS groups. Our results suggest antibacterial effect of 2% CC gel against E. faecalis in infected dentin, in addition to antibacterial substantivity of 2% CC and 2% CHX up to 7 days. (AU)

• Processo FAPESP: 13/23061-5 - Análise do conteúdo microbiológico e endotóxico de infecções endodônticas primarias e abscessos periapicais e sua relação com a sintomatologia clínica e os níveis de citocinas pró-inflamatórias, MMPs e Substância P
• Beneficiário: Daniel Rodrigo Herrera Morante
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado