| Texto completo | |
| Autor(es): |
Gomes-Cornelio, Ana Livia
;
Rodrigues, Elisandra Marcia
;
Mestieri, Leticia Boldrin
;
Rodrigues Sanzovo Falcoski, Thais de Oliveira
;
Soares, Christiane Pienna
;
Guerreiro-Tanomaru, Juliane Maria
;
Rossa Junior, Carlos
;
Tanomaru-Filho, Mario
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BRAZILIAN ORAL RESEARCH; v. 30, n. 1, p. 10-pg., 2016-01-01. |
| Resumo | |
Several calcium silicate-based biomaterials have been developed in recent years, in addition to Mineral Trioxide Aggregate (MTA). The aim of this study was to evaluate the cytotoxicity, genotoxicity and apoptosis/necrosis in human osteoblast cells (SAOS-2) of pure calcium silicate-based cements (CSC) and modified formulations: modified calcium silicate-based cements (CSCM) and three resin-based calcium silicate cements (CSCR1) (CSCR 2) (CSCR3). The following tests were performed after 24 hours of cement extract exposure: methyl-thiazolyl tetrazolium (MTT), apoptosis/necrosis assay and comet assay. The negative control (CT-) was performed with untreated cells, and the positive control (CT+) used hydrogen peroxide. The data for MTT and apoptosis were submitted to analysis of variance and Bonferroni's posttest (p < 0.05), and the data for the comet assay analysis, to the Kruskal-Wallis and Dunn tests (p < 0.05). The MTT test showed no significant difference among the materials in 2 mg/mL and 10 mg/mL concentrations. CSCR3 showed lower cell viability at 10 mg/mL. Only CSC showed lower cell viability at 50 mg/mL. CSCR1, CSCR2 and CSCR3 showed a higher percentage of initial apoptosis than the control in the apoptosis test, after 24 hours exposure. The same cements showed no genotoxicity in the concentration of 2 mg/mL, with the comet assay. CSC and CSCR2 were also not genotoxic at 10 mg/mL. All experimental materials showed viability with MTT. CSC and CSCR2 presented a better response to apoptosis and genotoxicity evaluation in the 10 mg/mL concentration, and demonstrated a considerable potential for use as reparative materials. (AU) | |
| Processo FAPESP: | 12/13268-9 - Viabilidade e bioatividade celular de materiais à base de cimento Portland em cultura primária de células do folículo e polpa dental humanos, fibroblastos e osteoblastos |
| Beneficiário: | Mario Tanomaru Filho |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 11/13116-1 - Avaliação da citotoxicidade, genotoxicidade e bioatividade de diferentes tipos de cimento Portland e associações radiopacificadores micro e nanoparticulados |
| Beneficiário: | Ana Lívia Gomes Cornélio |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 11/18239-4 - Avaliação físico-química, mecânica, de biocompatibilidade e bioatividade de diferentes formulações de cimento Portland e associações a agentes radiopacificadores micro e nanoparticulados |
| Beneficiário: | Mario Tanomaru Filho |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |