"Aroeira" (Myracrodruon urundeuva) methanol extract: the relationship between chemical compounds and cellular effects

Autor(es)

Machado, Alessandra Cury ; Souza, Leonardo Perez ; Saldanha, Luiz Leonardo ; Pieroni, Lais Goyos ; Matos, Adriana Arruda ; de Oliveira, Flavia Amadeu ; Vilegas, Wagner ; Damante, Carla Andreotti ; Dokkedal, Anne Ligia ; de Oliveira, Rodrigo Cardoso

• Tipo de documento: Artigo Científico
• Fonte: Pharmaceutical Biology; v. 54, n. 11, p. 5-pg., 2016-11-01.

Resumo

Context: Aroeira [Myracrodruon urundeuva AllemAo (Anacardiaceae)] is a tree whose leaves have been studied for therapeutic purposes in medicine and dentistry.Objective: The study chemically identifies the leaf extract of aroeira and determines its effect on human gingival fibroblasts.Materials and methods: An 80% methanol leave extract was obtained by maceration and chemically identified through flow-injection analysis-electrospray ionization-ion trap-tandem mass spectrometry (FIA-ESI-IT-MSn). Cytotoxicity of the aroeira's methanol extract was evaluated in lineage of fibroblasts. Adherent cells were treated with different concentrations of aroeira's methanol extract in the medium: 0.1, 1, 10, 100 and 1000g/mL. Control cells were cultivated in the medium only. Analyses were done at 24, 48, 72 and 96h of culture by neutral red assay; and at 24, 48 and 96h by crystal violet assay.Results: FIA-ESI-IT-MS analysis determined the presence of compounds, for the first time in the species: quercetin-O-glucuronide and quercetin-O-deoxyhexose-O-glucose in the extract. On one hand, neutral red and crystal violet assay showed a reduction (to 50% up until 100%) of cellular viability of groups of 100 and 1000g/mL compared with control at 96h (p<0.05). On the other hand, lower concentrations (0.1; 1 and 10g/mL) of the extract were similar to that of the control at 96h (p<0.05), in general.Conclusions: In view of the results, we can conclude that the extract of aroeira presents tannins and flavonoids. Furthermore, the extract is capable of modulating the viability of human gingival fibroblasts according to its concentration. (AU)

• Processo FAPESP: 11/22243-7 - Efeito do extrato de aroeira na adesão e proliferação de fibroblastos
• Beneficiário: Patricia Fernandes Sartori
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 10/14039-8 - Efeito do extrato de aroeira no processo de proliferação e mineralização de osteoblastos humanos in vitro
• Beneficiário: Adriana Arruda Matos
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 10/02026-9 - Avaliação da influência do extrato de aroeira na adesão e proliferação por fibroblatos humanos e expressão de MMP-2 e 9 in vitro
• Beneficiário: Rodrigo Cardoso de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular