An In Vitro Model for the Study of the Macrophage Response Upon Trichophyton rubrum Challenge

Autor(es)

Yamada Yoshikawa, Fabio Seiti ; Ferreira, Lucas Goncalves ; de Almeida, Fernando Goncalves ; de Almeida, Sandro Rogerio

• Tipo de documento: Artigo Científico
• Fonte: Mycopathologia; v. 182, n. 1-2, p. 10-pg., 2017-02-01.

Resumo

Diversity in the macrophage models currently employed in immunology studies may lead to opposed results and interpretations. In this study, we aimed to analyze the suitability of J774 macrophage-like cells as a model for the interaction between the dermatophyte Trichophyton rubrum and macrophages. J774 cells were competent in fungal phagocytosis, but succumbed to hyphal growth. Nevertheless, they could also secrete IL-1 beta in response to the dermatophyte. On the opposite direction, inflammatory, thioglycollate-induced peritoneal macrophages did not succumb to fungal growth and showed no significant IL-1 beta production. The proteomic profiling of these cells uncovered vimentin and plastin-2 as proteins whose abundance was altered by the fungal interaction. Our study indicates that this cell line could be an interesting tool in the investigation of T. rubrum infection biology. (AU)

• Processo FAPESP: 12/14684-6 - A participação do inflamassomo em modelo murino de infecção por Trichophyton rubrum: vias de ativação e relevância in vivo
• Beneficiário: Fábio Seiti Yamada Yoshikawa
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 10/07836-9 - Mecanismo de morte celular decorrente da interação entre macrófagos e conídios de Trichophyton rubrum
• Beneficiário: Fábio Seiti Yamada Yoshikawa
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica