Autor(es)

Giardini, Aline Carolina ; dos Santos, Fabio Martinez ; da Silva, Joyce Teixeira ; de Oliveira, Mara Evany ; Martins, Daniel Oliveira ; Chacur, Marucia

• Tipo de documento: Artigo Científico
• Fonte: PAIN RESEARCH & MANAGEMENT; v. 2017, p. 9-pg., 2017-01-01.

Resumo

Background. Glial cells are implicated in the development of chronic pain and brain-derived neurotropic factor ( BDNF) released from activated microglia contributes to the nociceptive transmission. Neural mobilization (NM) technique is a method clinically effective in reducing pain sensitivity. Here we examined the involvement of glial cells and BDNF expression in the thalamus and midbrain after NM treatment in rats with chronic constriction injury (CCI). CCI was induced and ratswere subsequently submitted to 10 sessions of NM, every other day, beginning 14 days after CCI. Thalamus and midbrain were analyzed for glial fibrillary acidic protein (GFAP), microglial cell OX-42, and BDNF using Immunohistochemistry and Western blot assays. Results. Thalamus and midbrain of CCI group showed increases in GFAP, OX-42, and BDNF expression compared with control group and, in contrast, showed decreases in GFAP, OX-42, and BDNF after NM when compared with CCI group. The decreased immunoreactivity for GFAP, OX-42, and BDNF in ventral posterolateral nucleus in thalamus and the periaqueductal gray in midbrain was shown by immunohistochemistry. Conclusions. These findings may improve the knowledge about the involvement of astrocytes, microglia, and BDNF in the chronic pain and show that NM treatment, which alleviates neuropathic pain, affects glial cells and BDNF expression. (AU)

• Processo FAPESP: 10/20026-6 - Uso do laser de baixa intensidade e os mecanismos celulares e moleculares no processo de reparação no sistema nervoso periférico
• Beneficiário: Daniel de Oliveira Martins
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 14/24533-0 - Terapia fotodinâmica na neuralgia do trigêmeo
• Beneficiário: Marucia Chacur
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/22268-0 - Aplicação do laser no controle da dor e na estimulação tecidual: da pesquisa básica à aplicação clínica
• Beneficiário: Marucia Chacur
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/05840-4 - Mobilização Neural: Avaliação molecular e comportamental em ratos Wistar após indução de dor neuropática
• Beneficiário: Fabio Martinez dos Santos
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/24408-6 - Ressonância magnética e ativação neuronal supraespinhal em modelo de dor neuropática crônica em ratos
• Beneficiário: Joyce Teixeira da Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto