Bacterial diversity of symptomatic primary endodontic infection by clonal analysis

Autor(es)

Menezes Nobrega, Leticia Maria ; Montagner, Francisco ; Ribeiro, Adriana Costa ; Pinto Mayer, Marcia Alves ; Figueiredo de Almeida Gomes, Brenda Paula

• Tipo de documento: Artigo Científico
• Fonte: BRAZILIAN ORAL RESEARCH; v. 30, n. 1, p. 9-pg., 2016-01-01.

Resumo

The aim of this study was to explore the bacterial diversity of 10 root canals with acute apical abscess using clonal analysis. Samples were collected from 10 patients and submitted to bacterial DNA isolation, 16S rRNA gene amplification, cloning, and sequencing. A bacterial genomic library was constructed and bacterial diversity was estimated. The mean number of taxa per canal was 15, ranging from 11 to 21. A total of 689 clones were analyzed and 76 phylotypes identified, of which 47 (61.84%) were different species and 29 (38.15%) were taxa reported as yet-uncultivable or as yet-uncharacterized species. Prevotella spp., Fusobacterium nucleatum, Filifactor alocis, and Peptostreptococcus stomatis were the most frequently detected species, followed by Dialister invisus, Phocaeicola abscessus, the uncharacterized Lachnospiraceae oral clone, Porphyromonas spp., and Parvimonas micra. Eight phyla were detected and the most frequently identified taxa belonged to the phylum Firmicutes (43.5%), followed by Bacteroidetes (22.5%) and Proteobacteria (13.2%). No species was detected in all studied samples and some species were identified in only one case. It was concluded that acute primary endodontic infection is characterized by wide bacterial diversity and a high intersubject variability was observed. Anaerobic Gram-negative bacteria belonging to the phylum Firmicutes, followed by Bacteroidetes, were the most frequently detected microorganisms. (AU)

• Processo FAPESP: 09/07760-5 - INVESTIGAÇÃO DA MICROBIOTA DE CANAIS RADICULARES INFECTADOS DE DENTES SINTIMÁTICOS E COM LESÃO PERIAPICAL POR CULTURA, CLONAGEM E SEQUENCIAMENTO DO GENE 16S rRNA.
• Beneficiário: Letícia Maria Menezes Nóbrega
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/09047-4 - Estudo da diversidade microbiana de infecções endodônticas primárias através de clonagem e sequenciamento do gene 16S rRNA
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Regular