| Texto completo | |
| Autor(es): |
Mizuno, Cassia Suemi
;
Ampomaah, Winnifred
;
Mendonca, Fernanda Ribeiro
;
Andrade, Gabriela Carvalho
;
Nazare da Silva, Ariel Maria
;
Goulart, Mirian Oliveira
;
dos Santos, Raquel Alves
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | GENETICS AND MOLECULAR BIOLOGY; v. 40, n. 3, p. 9-pg., 2017-07-01. |
| Resumo | |
The cytotoxicity and genotoxicity of the stilbenes (E)-methyl-4-(3-5-dimethoxystyryl) benzoate (ester), (E)-4-(3-5-dimethoxystyryl) aniline (amino), (Z)-1,3-dimethoxy-5-(4-methoxystyryl) benzene (cis-TMS) and (E)-1,3-dimethoxy5-(4-methoxystyryl) benzene (trans-TMS) were investigated in this work. Structural modifications of resveratrol, a naturally occurring stilbene, have been previously performed, including the replacement of hydroxyl by different functional groups. Such modifications resulted in significant improvement of target-specific effects on cell death and antiproliferative responses. The parameters were evaluated using XTT assay, clonogenic survival assay and the cytokinesis-block micronucleus assay in CHO-K1 and HepG2 cell lines. The results showed that cis-TMS is approximately 250-fold more cytotoxic than the amino and ester, and 128-fold more cytotoxic than trans-TMS. When genotoxicity was evaluated, only the trans-TMS did not significantly increase the frequency of micronucleus (MN). While the cis-TMS induced a mean of 5.2 and 5.9 MN/100 cells at 0.5 mu M in CHO-K1 and HepG2, respectively, the amino and ester induced 3.1 and 3.6 MN/100 cells at 10 mu M in CHO-K1, respectively, and 3.5 and 3.8 in HepG2. Trans-TMS is genotoxic only in HepG2 cells. Based on these results, the cis-TMS was the most cytotoxic and genotoxic compound in both cell lines. (AU) | |
| Processo FAPESP: | 14/12465-0 - Avaliação das vias de resposta a danos no DNA moduladas por estilbenos estruturalmente modificados |
| Beneficiário: | Raquel Alves dos Santos |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |