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TEMPERATURE, pH AND CARBON SOURCE AFFECT DRASTICALLY INDOLE ACETIC ACID PRODUCTION OF PLANT GROWTH PROMOTING YEASTS

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Autor(es):
Scarcella, A. S. de A. ; Bizarria Junior, R. ; Bastos, R. G. ; Magri, M. M. R.
Número total de Autores: 4
Tipo de documento: Artigo Científico
Fonte: Brazilian Journal of Chemical Engineering; v. 34, n. 2, p. 10-pg., 2017-04-01.
Resumo

Rhizosphere and phylloplane yeasts are able to produce phytohormones, acting as potential plant growth promoters. The aim of this study was to analyze the production of indole acetic acid (IAA) by yeasts using variations in the culture conditions. The yeasts evaluated were Rhodotorula mucilaginosa and Trichosporon asahii, which were cultivated in a potato broth medium with 20 g/L of glucose or sucrose as the carbon source. The cultivation was carried out at 22 degrees C, 25 degrees C or 30 degrees C, and the initial pH was adjusted to 3.0, 4.5 or 6.0. The cultures were analyzed using a colorimetric technique with Salkowski reagent. The cell count was determined in all of the conditions. The results for T. asahii showed that IAA production with sucrose was superior at pH 6.0, and that with glucose was superior at pH 4.5. These results indicate an association between the carbon source and medium pH. Rh. mucilaginosa (2F32) presented a high IAA production when compared with T. asahii (3S44 and 4C06) or even with other microorganisms referenced in the literature. The highest production (0.655 g/L) was obtained at pH 6.0 with glucose as the carbon source. Variations in the culture conditions did not affect the cell growth of the yeasts. (AU)

Processo FAPESP: 11/18676-5 - Caracterização de leveduras isoladas de áreas agrícolas na promoção de crescimento vegetal.
Beneficiário:Ana Sílvia de Almeida Scarcella
Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
Processo FAPESP: 13/02421-3 - Avaliação da interação de leveduras promotoras de crescimento vegetal com fungos micorrízicos e rizobactérias
Beneficiário:Rodolfo Bizarria Júnior
Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 11/02322-0 - Caracterização de leveduras isoladas de áreas agrícolas como promotoras de crescimento vegetal e na interação com fungos micorrízicos e rizobactérias
Beneficiário:Márcia Maria Rosa Magri
Modalidade de apoio: Auxílio à Pesquisa - Regular