| Texto completo | |
| Autor(es): |
Montellano Duran, Natalia
;
Spelzini, Dario
;
Wayllace, Natael
;
Boeris, Valeria
;
Barroso da Silva, Fernando L.
Número total de Autores: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | International Journal of Biological Macromolecules; v. 107, p. 8-pg., 2018-02-01. |
| Resumo | |
The interaction between quinoa proteins isolate (QP isolate) and the negatively charged polysaccharide iota-Carragennan (Carr) as a function of pH was studied. Experimental measurements as turbidity, hydrophobic surface, zeta-potential, and hydrodynamic size were carried out. Associative interaction between QP and Carr was found in the pH range between 1 and 2.9. When both molecules are negatively charged (pH > 5,5), a pure Coulombic repulsion regime is observed and the self-association of QP due to the Carr exclusion is proposed. In the intermediate pH range, the experimental data suggests that the charge regulation mechanism can overcome the electrostatic repulsion that may take place (and an attraction between QP and Carr can still be Observed). Computational simulations by means of free energy derivatives using the Monte Carlo method were carried out to better understand the interaction mechanism between QP and Carr. QP was modeled as a single protein using one of the major proteins, Chenopodin (Ch), and Carr was modeled as a negatively charged polyelectrolyte (NCP) chain, both in the cell model framework. Simulation results showed attractive interactions in agreement with the experimental data. (C) 2017 Elsevier B.V. All rights reserved. (AU) | |
| Processo FAPESP: | 15/16116-3 - Mecanismos moleculares de origem eletrostática responsáveis pela complexação de proteínas |
| Beneficiário: | Fernando Luis Barroso da Silva |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |