Engineering Complexity in Bacterial Regulatory Circuits for Biotechnological Applications

Autor(es)

Westmann, Caua Antunes ; Guazzaroni, Mara-Eugenia ; Silva-Rocha, Rafael

• Tipo de documento: Artigo Científico
• Fonte: MSYSTEMS; v. 3, n. 2, p. 4-pg., 2018-03-01.

Resumo

Engineering microbial systems allows the generation of new technologies having significant impact in the biotechnological industry and on human health. In the past few years, several synthetic biology approaches have been implemented in bacteria to allow precise engineering of novel regulatory circuits for several applications. The advent of high-throughput technologies and clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9-based DNA editing techniques have been pivotal in this process. Yet, despite the tremendous advances experienced recently, there are still a number of bottlenecks that need to be overcome in order to generate high-performance redesigned living machines, and the use of novel computer-aided approaches would be essential for this task. In this perspective, we discuss some of the main advances in the field of microbial engineering and the new technologies and approaches that should allow the construction of on demand synthetic microbial factories through the redesign of regulatory complexity. (AU)

• Processo FAPESP: 16/05472-6 - Engenharia e prospecção de novos elementos regulatórios em bactéria através de abordagens de biologia sintética
• Beneficiário: Cauã Antunes Westmann
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 12/22921-8 - Abordagens de biologia sintética para decifrar os mecanismos de integração de sinais em promotores bacterianos complexos
• Beneficiário: Rafael Silva Rocha
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 15/04309-1 - Novas abordagens para melhorar a prospecção funcional de biocatalizadores em bibliotecas metagenômicas
• Beneficiário: María Eugenia Guazzaroni
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores