Mitochondrial ribosome bL34 mutants present diminished translation of cytochrome c oxidase subunits

Autor(es)

Guedes-Monteiro, Raquel Fonseca ; Ferreira-Junior, Jose Ribamar ; Bleicher, Lucas ; Nobrega, Francisco G. ; Barrientos, Antoni ; Barros, Mario H.

• Tipo de documento: Artigo Científico
• Fonte: Cell Biology International; v. 42, n. 6, p. 13-pg., 2018-06-01.

Resumo

Saccharomyces cerevisiae mitoribosomes are specialized in the translation of a few number of highly hydrophobic membrane proteins, components of the oxidative phosphorylation system. Mitochondrial characteristics, such as the membrane system and its redox state driven mitoribosomes evolution through great diversion from their bacterial and cytosolic counterparts. Therefore, mitoribosome presents a considerable number of mitochondrial-specific proteins, as well as new protein extensions. In this work we characterize temperature sensitive mutants of the subunit bL34 present in the 54S large subunit. Although bL34 has bacterial homologs, in yeast it has a long 65 aminoacids mitochondrial N-terminal addressing sequence, here we demonstrate that it can be replaced by the mitochondrial addressing sequence of Neurospora crassa ATP9 gene. The bL34 temperature sensitive mutants present lowered translation of mitochondrial COX1 and COX3, which resulted in reduced cytochrome c oxidase activity and respiratory growth deficiency. The sedimentation properties of bL34 in sucrose gradients suggest that similarly to its bacterial homolog, bL34 is also a later participant in the process of mitoribosome biogenesis. (AU)

• Processo FAPESP: 13/09482-8 - Saccharomyces cerevisiae como modelo de estudo da tradução mitocondrial
• Beneficiário: Mario Henrique de Barros
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/18892-0 - Caracterização de produtos gênicos mitocondriais com função desconhecida em Saccharomyces cerevisiae.
• Beneficiário: Raquel Fonseca Guedes Monteiro
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/07937-8 - Redoxoma
• Beneficiário: Ohara Augusto
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 12/09762-8 - Estudo de proteínas mitocondriais de função desconhecida e suas consequências na viabilidade celular.
• Beneficiário: Ana Carolina Santana Santos
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico