Photobiomodulation of local alterations induced by BthTX-I, a phospholipase A(2) myotoxin from Bothrops jararacussu snake venom: In vivo and in vitro evaluation

Autor(es)

dos Santos, Adriano Silvio ; Guimaraes-Sousa, Ludmila ; Costa, Maricilia Silva ; Zamuner, Luis Fernando ; Sousa, Norma Cristina ; Hyslop, Stephen ; Soares, Andreimar M. ; Chavantes, Maria Cristina ; Cogo, Jose Carlos ; Zamuner, Stella Regina

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Biological Macromolecules; v. 107, p. 6-pg., 2018-02-01.

Resumo

This report describes the effect of photobiomodulation (PBM) on edema formation, leukocyte influx, prostaglandin E-2 (PGE(2)) biosynthesis and cytotoxicity caused by bothropstoxin-I (BthTX-I), a phospholipase A(2) (PLA(2)) homologue isolated from Bothrops jararacussu snake venom. Swiss mice or C2C12 cells were irradiated with low-level laser (LLL) at 685 nm wavelength, an energy density of 4.6 J/cm(2) and an irradiation time of 13 s. To evaluate the effect on edema formation and leukocyte influx, LLL was applied to the site of inoculation 30 min and 3 h post-injection. C2C12 cells were exposed to BthTX-I and immediately irradiated. PBM significantly reduced paw edema formation, peritoneal leukocyte influx and PGE(2) synthesis, but increased the viability of C2C12 muscle cells after BthTX-I incubation. These findings demonstrate that PBM attenuated the inflammatory events induced by BthTX-I. The attenuation of PGE(2) synthesis could be an important factor in the reduced inflammatory response caused by laser irradiation. The ability of LLL irradiation to protect muscle cells against the deleterious effects of BthTX-I may indicate preservation of the plasma membrane. (C) 2017 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 07/07137-0 - ESTUDO DA REAÇÃO INFLAMATÓRIA INDUZIDA PELO VENENO DA SERPENTE Bothrops jararacussu E POR DUAS MIOTOXINAS ISOLADAS DESTE VENENO
• Beneficiário: Ludmila Guimarães Souza
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 13/23757-0 - Efeito do laser de baixa potência sobre células musculares C2C12 submetidas à lesão por miotoxinas BthTX-I e BthTX-II isoladas do veneno da serpente Bothrops jararacussu
• Beneficiário: Adriano Silvio dos Santos
• Modalidade de apoio: Bolsas no Brasil - Mestrado