Isolation and characterization of a human cementocyte-like cell line, HCY-23

Autor(es)

de Almeida, Amanda Bandeira ; Lira dos Santos, Elis Janaina ; Abuna, Gabriel Flores ; Ribeiro, Cristiane Salmon ; Casati, Marcio Zaffalon ; Silverio Ruiz, Karina Gonzales ; Nociti Junior, Francisco Humberto

• Tipo de documento: Artigo Científico
• Fonte: BRAZILIAN ORAL RESEARCH; v. 33, p. 10-pg., 2019-01-01.

Resumo

Cementum is the mineralized tissue covering the tooth root that functions in tooth attachment and post-eruptive adjustment of tooth position. It has been reported to be highly similar to bone in several respects but remains poorly understood in terms of development and regeneration. Here, we investigate whether cementocytes, the residing cells in cellular cementum, have the potential to be protagonist in cementum homeostasis, responding to endocrine signals and directing local cementum metabolism. Cells from healthy erupted human teeth were isolated using sequential collagenase/EDTA digestions, and maintained in standard cell culture conditions. A cementocyte-like cell line was cloned (HCY-23, for human cementocyte clone 23), which presented a cementocyte compatible gene expression signature, including the expression of dentin matrix protein 1 (DMP1), sclerostin (SOST), and E11/gp38/podoplanin (E11). In contrast, these cells did not express the odontoblast/dentin marker dentin sialoprotein (DSPP). HCY-23 cells produced mineral-like nodules in vitro under differentiation conditions, and were highly responsive to inorganic phosphate (Pi). Within the limits of the present study, it can be concluded that cementocytes are phosphate-responsive cells, and have the potential do play a key role in periodontal homeostasis and regeneration. (AU)

• Processo FAPESP: 15/07232-0 - Isolamento e caracterização de uma linha de cementócitos
• Beneficiário: Amanda Bandeira de Almeida
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 15/06372-2 - Determinação do papel dos cementócitos na homeostasia do cemento dental
• Beneficiário: Francisco Humberto Nociti Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular