Autor(es)

Galetti, Renata ; Andrad, Leonardo Neves ; Varani, Alessandro M. ; Costa Darini, Ana Lucia

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE; v. 16, p. 4-pg., 2019-03-01.

Resumo

Objectives: The purpose of this study was to investigate the resistome of an SPM-1-producing Pseudomonas aeruginosa ST277 isolate (HC84) from Brazil. Methods: Whole-genome sequencing of P. aeruginosa HC84 was performed using an Ion Proton (TM) System. De novo assembly was carried out using CLC Genomics Workbench 8.0, and gene prediction was performed using the Prokka pipeline. Results and conclusion: Here we describe the resistome of SPM-1-producing P. aeruginosa ST277 (HC84) consisting of 13 different antimicrobial resistance genes [bla(SPM-1), rmtD, aacA4, aadA7, bla(OXA-56), bla(OXA-396), bla(PAO), aph(3 ')-IIb, aac(6 ')-Ib-cr, crpP, catB7, cmx and fosA). This particular chromosomal pack of resistance genes is strongly associated with clonal dissemination and suggests an important role in the persistence of this clone in Brazilian nosocomial infections. For that reason, could we already consider the 'chromosomal pack of acquired resistance genes' like an 'ST277 intrinsic resistome'? This is an example of chromosomal accumulation of acquired resistance genes as well as integrative and conjugative elements into a successful bacterial pathogen and calls attention to the evolution of other species driving to insertion and persistence of multiple acquired resistance genes in the bacterial chromosome. (C) 2018 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 15/11728-0 - Sequenciamento completo de plasmídeos carreando genes de resistência a antibióticos em bactérias de interesse e análise do genoma de linhagens de Pseudomonas aeruginosa produtoras de SPM-1 e de KPC-2 por sequenciamento em larga escala
• Beneficiário: Renata Galetti
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/14494-8 - Epidemiologia molecular de bactérias gram-negativas e genética da resistência a antibióticos
• Beneficiário: Ana Lúcia da Costa Darini
• Modalidade de apoio: Auxílio à Pesquisa - Temático