Reference genes for gene expression studies targeting sugarcane infected with Sugarcane mosaic virus (SCMV)

Autor(es)

da Silva, Marcel Fernando ; Goncalves, Marcos Cesar ; Brito, Michael dos Santos ; Nobile, Paula Macedo ; de Andrade, Larissa Mara ; Medeiros, Cibele Nataliane ; Creste, Silvana ; Pinto, Luciana Rossini

• Tipo de documento: Artigo Científico
• Fonte: BMC RESEARCH NOTES; v. 12, p. 7-pg., 2019-03-18.

Resumo

Objective: The selection of reference genes in sugarcane under Sugarcane mosaic virus (SCMV) infection has not been reported and is indispensable to get reliable reverse transcription quantitative PCR (RT-qPCR) results for validation of transcriptome analysis. In this regard, seven potential reference genes were tested by RT-qPCR and ranked according to their stability using BestKeeper, NormFinder and GeNorm algorithms, and RefFinder WEB-based software in an experiment performed with samples from two sugarcane cultivars contrasting for SCMV resistance, when mechanically inoculated with a severe SCMV strain and using mock inoculated plant controls. Results: The genes Uridylate kinase (UK) and Ubiquitin-conjugating enzyme 18 (UBC18) were the most stable according to GeNorm algorithm and the Pearson correlation coefficients with the BestKeeper index. On the other hand, ribosomal protein L35-4 (RPL1), Actin (ACT) and Ubiquitin1 (UBQ1) were the least stable genes for all algorithms tested. (AU)

• Processo FAPESP: 12/15060-6 - Identificação de genes candidatos para resistência ao mosaico da cana-de-açúcar a partir da técnica de cDNA-AFLP.
• Beneficiário: Cibele Nataliane Facioli Medeiros
• Modalidade de apoio: Bolsas no Brasil - Mestrado