Immobilization of Candida rugosa lipase on eco-friendly supports by spouted-bed technology: Use in the synthesis of isoamyl caprylate

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Costa-Silva, T. A. ; Carvalho, A. K. F. ; Souza, C. R. F. ; Freitas, L. ; De Castro, H. F. ; Oliveira, W. P. ; Carcel, JA ; Clemente, G ; GarciaPerez, JV ; Mulet, A ; Rossello, C

• Tipo de documento: Artigo Científico
• Fonte: IDS'2018: 21ST INTERNATIONAL DRYING SYMPOSIUM; v. N/A, p. 8-pg., 2018-01-01.

Resumo

Candida rugosa lipase (LCR) was immobilized on low-cost supports (byproducts) and dried using a spouted-bed system. The yields of immobilized derivatives were in the range 61.5-78.7%. Lipase immobilized on rice husk showed the best results, presenting 94.1% of the original activity, followed by sugarcane bagasse (90.3%) and green coconut fiber (87.3%). Moisture content in the obtained powders varied between 4.7 and 5.6% and the water activities were in the range 0.21-0.35. Among all the tested biocatalysts for aroma production the lipase immobilized on rice husk showed the highest activity towards the formation of isoamyl caprylate (62.40 g.L-1). (AU)

• Processo FAPESP: 11/00743-8 - Produção e imobilização de lipases produzidas pelo fungo endofítico Cercospora kikuchii para aplicações biotecnológicas
• Beneficiário: Tales Alexandre da Costa e Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado