Acute restraint stress increases blood pressure and oxidative stress in the cardiorenal system of rats: a role for AT(1) receptors

Autor(es)

do Vale, Gabriel T. ; Leoni, Drieli ; Sousa, Arthur H. ; Gonzaga, Natalia A. ; Uliana, Daniela L. ; La Gata, Davi C. ; Resstel, Leonardo B. ; Padovan, Claudia M. ; Tirapelli, Carlos R.

• Tipo de documento: Artigo Científico
• Fonte: STRESS-THE INTERNATIONAL JOURNAL ON THE BIOLOGY OF STRESS; v. 23, n. 3, p. 10-pg., 2019-10-14.

Resumo

We evaluate whether acute restraint stress may affect the oxidative state of the cardiorenal system and the possible contribution of angiotensin II/AT(1) receptors in such response. Male Wistar rats were restrained for 60?min within wire mesh chambers. Some rats were treated with losartan (selective AT(1) receptor antagonist, 10?mg/kg, p.o., gavage) 30?min before being stressed. Biochemical analyses were conducted after the 60-min period of restraint. Treatment with losartan prevented the increase in mean arterial pressure (MAP), but not heart rate (HR) induced by acute stress. Phenylephrine-induced contraction of endothelium-intact aortas was not affected by acute stress. Losartan prevented the increase in both superoxide anion (O-2(??)) and hydrogen peroxide (H2O2) levels induced by acute stress in the aorta and renal cortex. Similarly, the augmented activity of superoxide dismutase (SOD) induced by acute stress in the aorta and renal cortex was prevented by losartan. Enhanced levels of O-2(??) and thiobarbituric acid reactive species (TBARS) were detected in the left ventricle (LV) of stressed rats, but losartan did not prevent these responses. Similarly, losartan did not inhibited stress-induced decrease in the concentration of nitrate/nitrite (NOx) and H2O2 in the left ventricle. Stress increased ROS generation and affected the enzymatic antioxidant system in the cardiorenal system. In addition to its well-known cardiovascular changes during acute stress, angiotensin II also induces ROS generation in the cardiorenal system in a tissue-specific manner. The increase in oxidative stress mediated by angiotensin II/AT(1) receptors could be one mechanism by which acute stress predisposes to cardiorenal dysfunctions. (AU)

• Processo FAPESP: 15/22046-8 - Participação dos receptores AT1 no aumento da pressão arterial e do estresse oxidativo vascular induzidos pela abstinência ao etanol
• Beneficiário: Carlos Renato Tirapelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular