Multidrug-resistant CTX-M-15-positive Klebsiella pneumoniae ST307 causing urinary tract infection in a dog in Brazil

Autor(es)

Sartori, Luciana ; Sellera, Fabio P. ; Moura, Quezia ; Cardoso, Brenda ; Cerdeira, Louise ; Lincopan, Nilton

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE; v. 19, p. 2-pg., 2019-12-01.

Resumo

Objectives: Klebsiella pneumoniae sequence type 307 (ST307) has emerged as a new high-risk clone worldwide. The aim of this study was to report the draft genome sequence of a multidrug-resistant CTX-M-15-positive K. pneumoniae strain causing urinary tract infection in a companion animal in Brazil. Methods: Whole-genome sequencing was performed on an Illumina NextSeq platform and the genome was de novo assembled using SPAdes. Data analysis was performed using online tools from the Center for Genomic Epidemiology. Results: The genome size of K. pneumoniae strain PRETA was calculated at 5 450 575 bp, containing a total of 5355 protein-coding sequences. The strain was assigned to the high-risk ST307 and carried the clinically relevant bla(CTX-M-15) gene, besides other genes conferring resistance to aminoglycosides, tetracyclines, quinolones, trimethoprim and fosfomycin. Conclusion: These data offer novel information for comparative genomic analyses in order to track the transmission dynamics and epidemiology of the newly emerging high-risk clone ST307. (C) 2019 International Society for Antimicrobial Chemotherapy. Published by Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 16/08593-9 - Pan-resistoma de Klebsiella pneumoniae e Escherichia coli produtoras de beta-lactamases (KPC-2, CTX-M-8, CTX-M-15) endêmicas no Brasil
• Beneficiário: Nilton Erbet Lincopan Huenuman
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/13527-2 - Relação ancestral, pan-resistoma plasmidial de Escherichia coli produtora de b-lactamases de espectro estendido (CTX-M) e métodos de cura plasmidial
• Beneficiário: Miriam Rodriguez Fernandes
• Modalidade de apoio: Bolsas no Brasil - Doutorado