Herpesviruses in the oral cavity of patients subjected to allogeneic hematopoietic stem cell transplantation and its relationship with oral mucositis

Autor(es)

Miranda-Silva, Wanessa ; Knebel, Franciele Hinterholz ; Tozetto-Mendozo, Tania Regina ; Palmieri, Michelle ; da Fonseca, Felipe Paiva ; Camargo, Anamaria Aranha ; Braz-Silva, Paulo Henrique ; Fregnani, Eduardo Rodrigues

• Tipo de documento: Artigo Científico
• Fonte: CLINICAL ORAL INVESTIGATIONS; v. 24, n. 10, p. 12-pg., 2020-02-08.

Resumo

Objectives This study was performed to characterise oral shedding of herpesviruses in patients who underwent allogeneic hematopoietic stem cell transplantation (alloHSCT) and to investigate its relationship with oral mucositis (OM). Materials and Methods PCR and enzymatic digestion were conducted to identify oral shedding of herpesviruses and its correlation with OM development in 31 patients. The samples were collected at three sites in the oral cavity and at 5 times during follow-up; two additional collections were made from patients who developed ulcerative OM. Results HSV-1, EBV, CMV, HHV-6A, HHV-6B, and HHV-7 were detected in 4.97%, 16.02%, 4.41%, 2.20%, 3.31%, and 68% of the oral mucosal samples, respectively; 4.41%, 16.57%, 5.52%, 2.20%, 5.52%, and 63.53% of supragingival samples, respectively, and 4.41%, 18.23%, 2.76%, 1.65%, 2.75%, and 35.91% of subgingival samples, respectively. OM was diagnosed in 13 patients. The presence of HHV-7 in C1 (oral mucosa: p = 0.032) and C2 (supragingival: p = 0.009; subgingival: p = 0.002) was significantly increased in patients who developed OM, and patients exhibiting HHV-7 shedding in the oral cavity were 3.32-fold more likely to develop OM. Conclusions Patients who developed OM showed higher HHV-7 shedding in the oral cavity at nadir (immediately prior to OM development), suggesting modifications to the inflammatory microenvironment. (AU)

• Processo FAPESP: 15/07727-9 - Avaliação do ambiente imunoregulatório de lesões potencialmente malignas de boca, carcinomas de boca e orofaringe relacionados ou não ao HPV.
• Beneficiário: Paulo Henrique Braz da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular