Decreased Collagen Type I is Associated with Increased Metalloproteinase-2 Activity and Protein Expression of Leptin in the Myocardium of Obese Rats

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Tomaz da Silva-Bertani, Danielle Cristina ; Vileigas, Danielle Fernandes ; Ferreira Mota, Gustavo Augusto ; Borges de Souza, Sergio Luiz ; De Tomasi, Loreta Casquel ; Salome de Campos, Dijon Henrique ; de Deus, Adriana Fernandes ; Freire, Paula Paccielli ; Barnabe Alves, Carlos Augusto ; Padovani, Carlos Roberto ; Cicogna, Antonio Carlos

• Tipo de documento: Artigo Científico
• Fonte: Arquivos Brasileiros de Cardiologia; v. 115, n. 1, p. 9-pg., 2020-07-01.

Resumo

Background: Obesity is a risk factor for medical complications, including the cardiovascular system. There is limited information on collagen in the heart in obesity. Our previous study showed decreased protein levels of myocardial collagen type I in obese rats fed a high-fat diet for 34 weeks. However, the mechanisms responsible for low levels are not fully elucidated. Objective: The purpose of this study was to test the hypothesis that the reduction in collagen type I is associated with increased metalloproteinase-2 (MMP-2) activity, which is linked to elevated leptin in the myocardium of obese rats. Methods: Thirty-day-old male Wistar rats were randomized into two groups, control (standard diet) and obese (high-fat diet), and fed for 34 weeks. The general animal characteristics and metabolic and endocrine profiles were evaluated. Myocardial protein expressions of collagen I, leptin, tissue inhibitors of metalloproteinases (TIMP), and MMP-2 activity were assessed. Pearson correlation was employed to determine the associations between variables. The level of significance was 5%. Results: The obese animals had increased adiposity index compared to control. Comorbidities such as glucose intolerance, hyperinsulinemia, insulin resistance, hyperleptinemia, and hypertension were observed in obese rats. Obesity reduced collagen I, TIMP-1, and TIMP-2, and it increased leptin and MMP-2 in the myocardium. There was a negative correlation between collagen I and MMP-2 and a positive correlation between leptin and MMP-2. Conclusion: The hypothesis was confirmed; the reduction in collagen type I is associated with increased MMP-2 activity and leptin expression in the myocardium of obese rats. (AU)

• Processo FAPESP: 11/12165-9 - Influência da atividade das metaloproteinases 2 e 9 na diminuição do colágeno tipo I miocárdico em ratos obesos
• Beneficiário: Antonio Carlos Cicogna
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/14208-4 - Influência da atividade das metaloproteinases 2 e 9 na diminuição do colágeno tipo i miocárdico em ratos obesos.
• Beneficiário: Danielle Cristina Tomaz da Silva
• Modalidade de apoio: Bolsas no Brasil - Mestrado