Coexistence of the Oxazolidinone Resistance-Associated GenescfrandoptrAinEnterococcus faecalisFrom a Healthy Piglet in Brazil

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Almeida, Lara M. ; Gaca, Anthony ; Bispo, Paulo M. ; Lebreton, Francois ; Saavedra, Jose T. ; Silva, Rafael A. ; Basilio, Irinaldo D. ; Zorzi, Felipe M. ; Filsner, Pedro H. ; Moreno, Andrea M. ; Gilmore, Michael S.

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN PUBLIC HEALTH; v. 8, p. 8-pg., 2020-09-24.

Resumo

Oxazolidinones are one of the most important antimicrobials potentially active against glycopeptide- and beta-lactam-resistant Gram-positive pathogens. Linezolid-the first oxazolidinone to be approved for clinical use in 2000 by the US Food and Drug Administration-and the newer molecule in the class, tedizolid, inhibit protein synthesis by suppressing the formation of the 70S ribosomal complex in bacteria. Over the past two decades, transferable oxazolidinone resistance genes, in particularcfrandoptrA, have been identified in Firmicutes isolated from healthcare-related infections, livestock, and the environment. Our goals in this study were to investigate the genetic contexts and the transferability of thecfrandoptrAgenes and examine genomic features, such as antimicrobial resistance genes, plasmid incompatibility types, and CRISPR-Cas defenses of a linezolid-resistantEnterococcus faecalisisolated in feces from a healthy pig during an antimicrobial surveillance program for animal production in Brazil. Thecfrgene was found to be integrated into a transposon-like structure of 7,759 nt flanked by IS1216Eand capable of excising and circularizing, distinguishing it from known genetic contexts forcfrinEnterococcusspp., whileoptrAwas inserted into an Inc18 broad host-range plasmid of >58 kb. Conjugal transfer ofcfrandoptrAwas shown by filter mating. The coexistence ofcfrandoptrAin anE. faecalisisolated from a healthy nursery pig highlights the need for monitoring the use of antibiotics in the Brazilian swine production system for controlling spread and proliferation of antibiotic resistance. (AU)

• Processo FAPESP: 14/27267-0 - Análise dos efeitos de inibidores da recombinação homóloga em mutações ribossômicas envolvidas com a resistência à linezolida em cocos Gram-positivos
• Beneficiário: Lara Mendes de Almeida
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado