NCED expression is related to increased ABA biosynthesis and stomatal closure under aluminum stress

Autor(es)

Gavassi, Marina Alves ; Silva, Giselle Schwab ; Santiago da Silva, Carolina de Marchi ; Thompson, Andrew J. ; Macleod, Kyle ; Rayner Oliveira, Paulo Marcelo ; Cavalheiro, Mariana Feitosa ; Domingues, Douglas Silva ; Habermann, Gustavo

• Tipo de documento: Artigo Científico
• Fonte: ENVIRONMENTAL AND EXPERIMENTAL BOTANY; v. 185, p. 12-pg., 2021-02-06.

Resumo

Aluminum (Al)-induced decrease in leaf hydration has been associated with low gas exchange, especially stomatal conductance (g(s)). However, the mechanisms explaining these responses are unclear. Citrus limonia was exposed to 0 and 1480 mu M Al in nutrient solution for 90 days to test whether the low g(s) and leaf hydration in plants exposed to Al is associated with increased 9-cis-epoxycarotenoid dioxygenase (NCED) gene expression and abscisic acid (ABA) biosynthesis. Relative leaf water content (RWC), water potential (Psi w) and gas exchange in the leaves, as well as leaf and root ClNCED3, ClNCED1 and ClNCED5 expression and accumulation of ABA and its metabolites (phaseic acid, dihydrophaseic acid, (+)-7'-hydroxy-ABA and ABA-beta-D-glucosyl ester) were measured. Aluminum up-regulated ClNCED3 and induced ABA accumulation in the roots before impairments in leaf water status (low Psi w, RWC and g(s)) could be observed. Leaf ABA concentration increased from 7 to 90 days and this could be partially explained by the up-regulation of ClNCED3, ClNCED1 and ClNCED5 in this organ. Stomatal closure occurred concomitantly with the increase of ABA concentration, and this result provides further evidence of the role of ABA modulation of plant hydration under Al stress. (AU)

• Processo FAPESP: 16/10896-0 - A sinalização do ácido hexanóico pode modular o transcriptoma, metaboloma e o desenvolvimento de patógenos no cafeeiro?
• Beneficiário: Douglas Silva Domingues
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 18/08902-7 - Sinalização do ABA para o fechamento estomático sob estresse por Al
• Beneficiário: Marina Alves Gavassi
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 17/26144-0 - Aspectos hidráulicos, metabólicos e moleculares da interferência do Al3+ na hidratação da parte aérea em Citrus limonia
• Beneficiário: Gustavo Habermann
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/25658-2 - Interferência do alumínio na hidratação da parte aérea em Citrus limonia e no desempenho fotossintético em Vochysia tucanorum
• Beneficiário: Giselle Schwab Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/25409-4 - Papel do ABA nas relações hídricas em metabolismos contrastantes à sensibilidade ao Al3+
• Beneficiário: Marina Alves Gavassi
• Modalidade de apoio: Bolsas no Brasil - Doutorado