Comparison of Clamping Modalities in a Rabbit Model of Normothermic Renal Ischemia

Autor(es)

Formiga, Cipriano C. ^[1] ; Mitre, Anuar I. ^[1] ; Castilho, Lisias N. ^[2] ; Buchpiguel, Carlos Alberto ^[3] ; Marques, Fabio L. N. ^[3] ; Radin, Adriano ; Srougi, Miguel ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med, Dept Urol, BR-05403000 Sao Paulo - Brazil

^[2] Pontificia Univ Catolica Campinas, Dept Urol, Campinas, SP - Brazil

^[3] Univ Sao Paulo, Sch Med, Dept Radiol, Div Nucl Med, BR-05403000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ENDOUROLOGY; v. 25, n. 11, p. 1787-1791, NOV 2011.
• Citações Web of Science: 6

Resumo

Purpose: The objective of this study was to investigate the patterns of renal function recovery with different renal vessel clamping modalities during a prolonged warm ischemia (WI) condition in an experimental two-kidney rabbit model. Materials and Methods: Twenty-eight rabbits were randomly clustered into four groups and underwent laparotomy with different types of renal pedicle clamping. Group 1 (n = 4) was sham-operated. Group 2 (n = 8) underwent 80 minutes of WI with artery only clamping. Group 3 (n = 8) underwent arteriovenous clamping for 80 minutes, and group 4 (n = 8) received an arteriovenous clamping for 80 minutes with 10-second declamping periods every 20 minutes. Serum levels of creatinine (SCr) were recorded preoperatively and on postoperative days (PODs) 1, 3, and 7. Renal function was evaluated by (99m)technetium-mercaptoacetyltriglycine scintigraphy. Afterward, the animals were euthanized, and the kidneys were harvested and evaluated microscopically. Results: Renal function completely recuperated on POD 7 in the groups that underwent artery only and ateriovenous intermittent clamping, and both of these methods were superior to ateriovenous clamping (P < 0.001). SCr showed a similar variation in all the clamping groups and did not demonstrate statistical differences among the groups. Histopathologic changes were similar among the ischemic groups. Conclusion: The less deleterious clamping modalities in this experimental model were the artery only and intermittent en bloc clamping methods. (AU)

• Processo FAPESP: 08/58420-7 - Repercussões morfológicas e funcionais em parênquima renal de coelho submetido à isquemia: comparação entre modalidades de isquemia seletiva e não-seletiva
• Beneficiário: Anuar Ibrahim Mitre
• Modalidade de apoio: Auxílio à Pesquisa - Regular