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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Comparison of Clamping Modalities in a Rabbit Model of Normothermic Renal Ischemia

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Author(s):
Formiga, Cipriano C. [1] ; Mitre, Anuar I. [1] ; Castilho, Lisias N. [2] ; Buchpiguel, Carlos Alberto [3] ; Marques, Fabio L. N. [3] ; Radin, Adriano ; Srougi, Miguel [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Sch Med, Dept Urol, BR-05403000 Sao Paulo - Brazil
[2] Pontificia Univ Catolica Campinas, Dept Urol, Campinas, SP - Brazil
[3] Univ Sao Paulo, Sch Med, Dept Radiol, Div Nucl Med, BR-05403000 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF ENDOUROLOGY; v. 25, n. 11, p. 1787-1791, NOV 2011.
Web of Science Citations: 6
Abstract

Purpose: The objective of this study was to investigate the patterns of renal function recovery with different renal vessel clamping modalities during a prolonged warm ischemia (WI) condition in an experimental two-kidney rabbit model. Materials and Methods: Twenty-eight rabbits were randomly clustered into four groups and underwent laparotomy with different types of renal pedicle clamping. Group 1 (n = 4) was sham-operated. Group 2 (n = 8) underwent 80 minutes of WI with artery only clamping. Group 3 (n = 8) underwent arteriovenous clamping for 80 minutes, and group 4 (n = 8) received an arteriovenous clamping for 80 minutes with 10-second declamping periods every 20 minutes. Serum levels of creatinine (SCr) were recorded preoperatively and on postoperative days (PODs) 1, 3, and 7. Renal function was evaluated by (99m)technetium-mercaptoacetyltriglycine scintigraphy. Afterward, the animals were euthanized, and the kidneys were harvested and evaluated microscopically. Results: Renal function completely recuperated on POD 7 in the groups that underwent artery only and ateriovenous intermittent clamping, and both of these methods were superior to ateriovenous clamping (P < 0.001). SCr showed a similar variation in all the clamping groups and did not demonstrate statistical differences among the groups. Histopathologic changes were similar among the ischemic groups. Conclusion: The less deleterious clamping modalities in this experimental model were the artery only and intermittent en bloc clamping methods. (AU)