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Evaluation of NGAL as an early marker of renal damage in rats submitted to ischemia renal under total intravenous anesthesia with ringer lactate starch or: comparison with the discretion of Rifle

Grant number: 10/07799-6
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): July 01, 2010
Effective date (End): June 30, 2012
Field of knowledge:Health Sciences - Medicine - Surgery
Principal researcher:Norma Sueli Pinheiro Módolo
Grantee:Mariana Ferreira de Lima
Home Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

20 Wistar rats weighing 250-300 grams are anesthetized with propofol and remifentanil by continuous infusion to achieve D nephrectomy and renal artery clamping and for a period of 30 minutes. The animals will be divided into two study groups according to volume replacement performed: Ringer's lactate or hydroxyethyl starch. Blood samples taken before the completion of nephrectomy and renal ischemia after releasing the clamp, 30 minutes and in the immediate postoperative period for assay of creatinine and NGAL.These doses will be compared to the criterion of Rifle. (AU)

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