Effects determined by the administration of intrathecal dexmedetomidine in single Puncture on the spinal cord and meninges of rabbits

• Grant number: 11/22262-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2012
• End date: December 31, 2012
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Eliana Marisa Ganem
• Grantee: Natália de Castro Fim Nakao
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

ABSTRACTBACKGROUND AND PURPOSE: Hemodynamic changes lead to perioperative renal hypoperfusion with consequent failure of the organ. Ischemia and reperfusion lesion is the primary cause of acute renal failure in this period, increasing the morbidity and mortality. Dexmedetomidine is a selective ±2 agonist that improves hemodynamic stability during surgery. Its effect on renal function was studied in cases of hemorrhage. This research objective is to study the effects of dexmedetomidine on renal function in a model of ischemia and reperfusion in rats. METHODS: Thirty adult rats will be randomized into three groups: Group S or Sham (n = 10) anesthetized with isoflurane and dexmedetomidine (3mcg/Kg in 10 minutes, followed by 3mcg/Kg/h) and submitted to laparotomy with right nephrectomy; Group C or control (n = 10) anesthetized with isoflurane and submitted to laparotomy and right nephrectomy with left renal artery clamping for 30 minutes followed by reperfusion for 30 minutes; Study Group E (n = 10) anesthetized with isoflurano and dexmedetomidine (3mcg/Kg in 10 minutes, followed by 3mcg/Kg/h) laparotomy and right nephrectomy with left renal artery clamping for 30 minutes followed reperfusion for 30 minutes. Hemodynamic variables (heart rate and blood pressure), temperature and biochemical markers of renal function (NGAL, creatinine and interleukins) will be studied immediately after stabilization (M1), thirty minutes after left renal artery clamping (M2), thirty minutes after renal reperfusion (M3) and 24 hours after reperfusion (M4). KEY WORDS: Dexmedetomidine, kidney, renal protection, Ischemia and reperfusion.