Evaluation of caries-affected dentin with optical coherence tomography

Autor(es)

Cynthia Soares de Azevedo ^[1] ; Luciana Cardoso Espejo Trung ^[2] ; Maria Regina Lorenzetti Simionato ^[3] ; Anderson Zanardi de Freitas ^[4] ; Adriana Bona Matos ^[5]

Afiliação do(s) autor(es)

^[1] University of São Paulo. Dental School. Department of Operative Dentistry - Brasil

^[2] University of São Paulo. Dental School. Department of Operative Dentistry - Brasil

^[3] University of São Paulo. Institute of Biomedical Sciences. Department of Microbiology - Brasil

^[4] Nuclear and Energy Research Institute - Brasil

^[5] University of São Paulo. Dental School. Department of Operative Dentistry - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Oral Research; v. 25, n. 5, p. 407-413, 2011-10-00.

Resumo

The purpose of this study was to evaluate the degree of demineralization of artificially induced caries-affected human dentin by an in vitro microbiological method. The occlusal surfaces of 6 human molar teeth were abraded until a flat surface was obtained, and the enamel was removed to expose the occlusal dentin surface. These teeth were sectioned in 12 halves in the vestibular-lingual direction and divided into 3 groups according to the period length of the microbiological essay (n = 4): G1, 7 days; G2, 14 days; and G3, 21 days. The surfaces of all specimens were protected by an acid-resistant nail varnish, except for a window where the caries lesion was induced by a Streptoccocus mutans biofilm in a batch-culture model supplemented with 5% sucrose. The specimens were then analyzed by optical coherence tomography (OCT) with a super-luminescent light diode (Λ = 930 nm) with 6.0-µm lateral and longitudinal resolution (in the air). Qualitative and quantitative results (images and average dentin demineralization, respectively) were obtained. The mean demineralization depths were (µm) 235 ± 31.4, 279 ± 14, and 271 ± 8.3 in groups 1, 2, and 3, respectively. In addition, no significant change was observed in the lesion mean depth from 7 days of cariogenic challenge on. In conclusion, OCT was shown to be an efficient and non-invasive method to detect the depths of lesions caused by demineralization. Further, a seven-day demineralization time was considered sufficient for caries-affected dentin to be obtained. (AU)

• Processo FAPESP: 09/07709-0 - Estudo de adesão em dentina remanescente após remoção de tecido cariado com lasers de alta intensidade
• Beneficiário: Cynthia Soares de Azevedo
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica