| Texto completo | |
| Autor(es): |
Lobo, A. O.
[1, 2]
;
Marciano, F. R.
[1, 2]
;
Ramos, S. C.
[3]
;
Machado, M. M.
[4]
;
Corat, E. J.
[3]
;
Corat, M. A. F.
[4]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Vale Paraiba UniVap, IP&D, Lab Biomed Nanotechnol NanoBio, BR-12244000 Sao Jose Dos Campos, SP - Brazil
[2] Univ Vale Paraiba UniVap, IP&D, Lab Biomed Vibrat Spect LEVB, BR-12244000 Sao Jose Dos Campos, SP - Brazil
[3] INPE, Lab Associado Sensores & Mat LAS, BR-12245970 Sao Jose Dos Campos, SP - Brazil
[4] Univ Estadual Campinas UNICAMP, Ctr Multidisciplinar Invest Biol, Area Ciencia Anim Lab CEMIB, BR-13083877 Campinas, SP - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Materials Science & Engineering C-Materials for Biological Applications; v. 31, n. 7, p. 1505-1511, OCT 10 2011. |
| Citações Web of Science: | 16 |
| Resumo | |
We have analyzed the adhesion of mouse embryonic fibroblasts (MEFs) genetically modified by green fluorescence protein (GFP) gene cultured on vertically-aligned carbon nanotubes (VACNTs) after 6 days. The VACNTs films grown on Ti were obtained by microwave plasma chemical vapor deposition process using Fe catalyst and submitted to an oxygen plasma treatment, for 2 min, at 400 V and 80 mTorr, to convert them to superhydrophilic. Cellular adhesion and morphology were analyzed by scanning electron, fluorescence microscopy, and thermodynamics analysis. Characterizations of superhydrophilic VACNTs films were evaluated by contact angle and X-Ray Photoelectron Spectroscopy. Differences of crowd adhered cells, as well as their spreading on superhydrophilic VACNTs scaffolds, were evaluated using focal adhesion analysis. This study was the first to demonstrate, in real rime, that the wettability of VACNTs scaffolds might have enhanced and differential adherence patterns to the MEF-GFP on VACNTs substrates. (C) 2011 Elsevier B.V. All rights reserved. (AU) | |
| Processo FAPESP: | 07/00013-4 - Novos materiais, estudos e aplicações inovadoras em diamante-CVD, diamond-like-carbon (DLC) e carbono nanoestruturado obtidos por deposição química a partir da fase vapor |
| Beneficiário: | Evaldo Jose Corat |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 06/03525-3 - Sintese de nanotubos de carbono para aplicacoes tribologicas e estruturais. |
| Beneficiário: | Erica Freire Antunes |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |