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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Increasing mouse embryonic fibroblast cells adhesion on superhydrophilic vertically aligned carbon nanotube films

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Author(s):
Lobo, A. O. [1, 2] ; Marciano, F. R. [1, 2] ; Ramos, S. C. [3] ; Machado, M. M. [4] ; Corat, E. J. [3] ; Corat, M. A. F. [4]
Total Authors: 6
Affiliation:
[1] Univ Vale Paraiba UniVap, IP&D, Lab Biomed Nanotechnol NanoBio, BR-12244000 Sao Jose Dos Campos, SP - Brazil
[2] Univ Vale Paraiba UniVap, IP&D, Lab Biomed Vibrat Spect LEVB, BR-12244000 Sao Jose Dos Campos, SP - Brazil
[3] INPE, Lab Associado Sensores & Mat LAS, BR-12245970 Sao Jose Dos Campos, SP - Brazil
[4] Univ Estadual Campinas UNICAMP, Ctr Multidisciplinar Invest Biol, Area Ciencia Anim Lab CEMIB, BR-13083877 Campinas, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Materials Science & Engineering C-Materials for Biological Applications; v. 31, n. 7, p. 1505-1511, OCT 10 2011.
Web of Science Citations: 16
Abstract

We have analyzed the adhesion of mouse embryonic fibroblasts (MEFs) genetically modified by green fluorescence protein (GFP) gene cultured on vertically-aligned carbon nanotubes (VACNTs) after 6 days. The VACNTs films grown on Ti were obtained by microwave plasma chemical vapor deposition process using Fe catalyst and submitted to an oxygen plasma treatment, for 2 min, at 400 V and 80 mTorr, to convert them to superhydrophilic. Cellular adhesion and morphology were analyzed by scanning electron, fluorescence microscopy, and thermodynamics analysis. Characterizations of superhydrophilic VACNTs films were evaluated by contact angle and X-Ray Photoelectron Spectroscopy. Differences of crowd adhered cells, as well as their spreading on superhydrophilic VACNTs scaffolds, were evaluated using focal adhesion analysis. This study was the first to demonstrate, in real rime, that the wettability of VACNTs scaffolds might have enhanced and differential adherence patterns to the MEF-GFP on VACNTs substrates. (C) 2011 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 07/00013-4 - New materials, studies and applications of CVD diamond, diamond-like-carbon (DLC) and nanostructured carbon obtained by chemical vapor deposition
Grantee:Evaldo Jose Corat
Support Opportunities: Research Projects - Thematic Grants