Development of new polymeric scaffolds by electrospinning technique with incorporation of vertically aligned carbon nanotubes and nanohidroxyapatite for bone tissue regeneration

• Grant number: 11/17877-7
• Support Opportunities: Research Grants - Young Investigators Grants
• Duration: February 01, 2012 - September 30, 2016
• Field of knowledge: Engineering - Materials and Metallurgical Engineering - Nonmetallic Materials
• Principal Investigator: Anderson de Oliveira Lobo
• Grantee: Anderson de Oliveira Lobo
• Host Institution: Instituto de Pesquisa e Desenvolvimento (IP&D). Universidade do Vale do Paraíba (UNIVAP). São José dos Campos , SP, Brazil
• Associated researchers: Airton Abrahao Martin ; Cristina Pacheco Soares ; Emilia Angela Lo Schiavo Arisawa ; Erica Freire Antunes ; Evaldo Jose Corat ; Fernanda Roberta Marciano ; Marcus Alexandre Finzi Corat ; Marimélia Aparecida Porcionatto ; Rosario Elida Suman Bretas ; Vladimir Jesus Trava-Airoldi
• Associated grant(s): 14/50720-2 - Novel nanobiomaterials based on tridimensional peptides with drug delivery properties to tissue engineering, AP.R
• Associated scholarship(s): 16/10318-6 - Production and characterization of electrochemical nanosensors of polypyrrole for detection of heavy metals, BP.IC; 15/09697-0 - Electrospinning of novel functional nanobiomaterials based on peptides and bottlebrush polymers for tissue engineering, BE.PQ; 16/01584-4 - Hydrogels of amniotic membrane and poly(ethylene glycol) (PEG) to the regeneration cartilaginous tissue, BP.MS; + associated scholarships 16/04633-6 - Dispersion of carbon nanotubes in polymer solutions for the electrospinning technique use, BP.IC; 15/07904-8 - Bioactive scaffolds of poly(butilene adipate-co- terephtalate)to biomedical application: in vitro study, BP.IC; 15/08523-8 - Development of a fluorescent nanosensor from the electrospinning of PVA and graphene quantum dots: application in the detection of Alzheimer's biomarkers, BP.PD; 15/01259-3 - Electrospinning by solution of PBAT built nanohidroxiapatita, BP.IC; 14/16295-2 - Nanofibers produced by electrospinning and rotary jet-spinning with incorporated hydroxyapatite and carbon nanotubes to verify its potential in bone regeneration, BP.DR; 14/20561-0 - Bioreabsorbable polymeric nanodevice as peptide controlled delivery system to Alzheimer's disease therapy, BP.DD; 13/08341-1 - Electrospinning of polymeric nanofibers of poly-acid lactic with incorporated titanium dioxide nanoparticles, BP.IC; 13/07696-0 - Production of polymeric scaffolds by rotary-jet-spinning technique with incorporated carbon nanotubes, BP.IC; 12/19371-6 - Production of nanobiomaterials based on nanohydroxyapatite/carbon nanotubes and polycaproloactone, BP.IC; 12/15773-2 - Direct hydroxyapatite electrodeposition on superhydrophilic vertically aligned carbon nanotube films as scaffolds to bone tissue growth, BP.IC; 12/02159-4 - In vitro analysis of the biomineralization and cell proliferation on nanohydroxyapatite/vertically aligned carbon nanotube scaffolds, BP.IC - associated scholarships

Abstract

This project presents a technological innovation for polymer scaffold fabrication with the incorporation of nanoparticles by electrospinning technique for bone tissue regeneration. Superhydrophilic vertical aligned carbon nanotube (VACNT) matrix will be used with nanohydroxiapatite (nHA) crystals obtained by electrodeposition method. The fabrication of tridimensional polymeric nanofibrous scaffolds is proposed with the homogeneous exposure of nHA crystals for bone growth. Associated to this methodology, different kind of nanoparticles (such as TiO2 and nanocrystalline diamond particles, NCD) of bone-healing interest is going to be dispersed in polymeric matrices. VACNTs will be produced using two techniques (thermal and chemical vapor deposition assisted by microwave plasma) and functionalizated by physical and chemical processes in order to obtain their functionalization. Electrodeposition technique will be used to obtain homogeneous nHA crystals over superhydrofilic VACNTs. Polymeric nanofibrous scaffolds will be fabricated using eletrospinning technique. Bioresorbable polymers are used as matrices and TiO2, NCD and nHA as nanocharges. Scanning electron microscopy, transmission electron microscopy, X-ray difractometry, X-ray dispersive spectroscopy and optical spectroscopy will be used to morphological and structural characterization. For biological characterization will be used in vitro tests using: (i) Human osteoblast cell culture, (ii) mesenchimal steam cell and (iii) rats embriogenic genetic cell. In vivo tests will be carried out using rats. For this methodology will be realize bone defects and the polymeric nanocomposites produced will be inserted. Biological characterization will be carried out using: (i) colorimetric cytotoxicity assays, (ii) cytocompatibility using immunocytochemical and scanning electron microscopy technique; (iii) In vitro bioactivity, biomineralization and osteogenesis assays; (iv) Real time immigration of mesenchimal steam cell; (v) In vitro migration and recruitment of mesenchimal steam cell; (vi) In vivo assay in rats bone defect; (vii) In vivo osteointegration process study using Raman spectroscopy technique. We will be expecting that this new nanobiomaterial accelerate the osteointegration in vivo process. (AU)

Scientific publications

(76)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARSI, TERESA CRISTINA O.; SANTOS, TIAGO G.; PACHECO-SOARES, CRISTINA; CORAT, EVALDO J.; MARCIANO, FERNANDA R.; LOBO, ANDERSON O.. Biomineralization of Superhydrophilic Vertically Aligned Carbon Nanotubes. Langmuir, v. 28, n. 9, p. 4413-4424, MAR 6 2012. (11/17877-7)

ANTONIOLI, ELIANE; LOBO, ANDERSON O.; FERRETTI, MARIO; COHEN, MOISES; MARCIANO, FERNANDA R.; CORAT, EVALDO J.; TRAVA-AIROLDI, VLADIMIR J.. An evaluation of chondrocyte morphology and gene expression on superhydrophilic vertically-aligned multi-walled carbon nanotube films. Materials Science & Engineering C-Materials for Biological Applications, v. 33, n. 2, p. 641-647, MAR 1 2013. (11/20345-7, 11/17877-7)

LOBO, ANDERSON OLIVEIRA; SIQUEIRA, IDALIA A. W. B.; DAS NEVES, MARCELE F.; MARCIANO, FERNANDA ROBERTA; CORAT, EVALDO JOSE; CORAT, MARCUS ALEXANDRE F.. In vitro and in vivo studies of a novel nanohydroxyapatite/superhydrophilic vertically aligned carbon nanotube nanocomposites. JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, v. 24, n. 7, p. 1723-1732, JUL 2013. (11/20345-7, 11/17877-7)

BARBOSA, MICHELLE C.; MESSMER, NIGEL R.; BRAZIL, TAYRA R.; MARCIANO, FERNANDA R.; LOBO, ANDERSON O.. The effect of ultrasonic irradiation on the crystallinity of nano-hydroxyapatite produced via the wet chemical method. Materials Science & Engineering C-Materials for Biological Applications, v. 33, n. 5, p. 2620-2625, JUL 1 2013. (11/20345-7, 11/17877-7)

NEVES, MARCELE FLORENCIO; SILVA, GISLENE RODRIGUES; BRAZIL, TAYRA RODRIGUES; MARCIANO, FERNANDA ROBERTA; PACHECO-SOARES, CRISTINA; LOBO, ANDERSON OLIVEIRA. Calcification in vitro of biomineralizated nanohydroxyapatite/superydrophilic vertically aligned multiwalled carbon nanotube scaffolds. MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS, v. 16, n. 3, p. 614-618, MAY-JUN 2013. (11/20345-7, 12/02159-4, 11/17877-7)

NEVES, MARCELE FLORENCIO; BRAZIL, TAYRA RODRIGUES; SILVA SOARES, LUIS EDUARDO; CORAT, EVALDO JOSE; MARCIANO, FERNANDA ROBERTA; LOBO, ANDERSON OLIVEIRA. In vitro biomineralization of a novel hydroxyapatite/superhydrophilic multiwalled carbon nanotube nanocomposite using simulated body fluids. MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS, v. 16, n. 3, p. 650-654, MAY-JUN 2013. (11/20345-7, 12/02159-4, 11/17877-7)

PEREIRA, F. A. S.; SALLES, G. N.; RODRIGUES, B. V. M.; MARCIANO, F. R.; PACHECO-SOARES, C.; LOBO, A. O.. Diamond nanoparticles into poly(lactic acid) electrospun fibers: Cytocompatible and bioactive scaffolds with enhanced wettability and cell adhesion. Materials Letters, v. 183, p. 420-424, NOV 15 2016. (11/20345-7, 14/20561-0, 15/08523-8, 11/17877-7)

LOPES, F. S.; OLIVEIRA, J. R.; MILANI, J.; OLIVEIRA, L. D.; MACHADO, J. P. B.; TRAVA-AIROLDI, V. J.; LOBO, A. O.; MARCIANO, F. R.. Biomineralized diamond-like carbon films with incorporated titanium dioxide nanoparticles improved bioactivity properties and reduced biofilm formation. Materials Science & Engineering C-Materials for Biological Applications, v. 81, p. 373-379, DEC 1 2017. (12/15857-1, 11/17877-7, 11/20345-7, 14/11491-8, 15/09697-0, 16/00575-1)

RODRIGUES, BRUNO V. M.; CABRAL, TAYNA S.; SGOBBI, LIVIA F.; DELEZUK, JORGE A. M.; PESSOA, RODRIGO S.; TRIBONI, EDUARDO R.; DE MORAES, THAISA B. F.; LOBO, ANDERSON O.. A simple and green method for the production of nanostructured materials from poly(vinyl alcohol)/graphene quantum dots. Materials Chemistry and Physics, v. 219, p. 242-250, NOV 1 2018. (11/17877-7, 13/08166-5, 15/08523-8, 17/13839-0, 17/18826-3)

A. L. M. MAIA F.; J. L. DA SILVA; F. P. M. DO AMARAL; A. A. MARTIN; A. O. LOBO; L. E. S. SOARES. Avaliação morfológica e química do osso com implantes de Al2O3 recobertos com apatita como suporte para a reparação óssea. Cerâmica, v. 59, n. 352, p. 533-538, 2013-12-00. (11/17877-7)

DE CASTRO, JUCARA G.; RODRIGUES, BRUNO V. M.; RICCI, RITCHELLI; COSTA, MAIRA M.; RIBEIRO, ANDRE F. C.; MARCIANO, FERNANDA R.; LOBO, ANDERSON O.. Designing a novel nanocomposite for bone tissue engineering using electrospun conductive PBAT/polypyrrole as a scaffold to direct nanohydroxyapatite electrodeposition. RSC ADVANCES, v. 6, n. 39, p. 32615-32623, 2016. (11/20345-7, 15/08523-8, 11/17877-7)

HOLLANDA, L. M.; LOBO, A. O.; LANCELLOTTI, M.; BERNI, E.; CORAT, E. J.; ZANIN, H.. Graphene and carbon nanotube nanocomposite for gene transfection. Materials Science & Engineering C-Materials for Biological Applications, v. 39, p. 288-298, JUN 1 2014. (11/17195-3, 11/17877-7)

TONIATTO, T. V.; RODRIGUES, B. V. M.; MARSI, T. C. O.; RICCI, R.; MARCIANO, F. R.; WEBSTER, T. J.; LOBO, A. O.. Nanostructured poly (lactic acid) electrospun fiber with high loadings of TiO2 nanoparticles: Insights into bactericidal activity and cell viability. Materials Science & Engineering C-Materials for Biological Applications, v. 71, p. 381-385, FEB 1 2017. (11/17877-7, 15/08523-8, 13/08341-1, 11/20345-7, 14/16966-4)

SIQUEIRA, L.; PASSADOR, F. R.; COSTA, M. M.; LOBO, A. O.; SOUSA, E.. Influence of the addition of beta-TCP on the morphology, thermal properties and cell viability of poly (lactic acid) fibers obtained by electrospinning. Materials Science & Engineering C-Materials for Biological Applications, v. 52, p. 135-143, JUL 1 2015. (11/17877-7)

MACHADO, M. M.; LOBO, A. O.; MARCIANO, F. R.; CORAT, E. J.; CORAT, M. A. F.. Analysis of cellular adhesion on superhydrophobic and superhydrophilic vertically aligned carbon nanotube scaffolds. Materials Science & Engineering C-Materials for Biological Applications, v. 48, p. 365-371, MAR 1 2015. (11/20345-7, 11/17877-7)

SOUZA, ROBERTA K. F.; AZEVEDO, ROSANA F. F.; LOBO, ANDERSON O.; RANGEL, DRAUZIO E. N.. Conidial water affinity is an important characteristic for thermotolerance in entomopathogenic fungi. BIOCONTROL SCIENCE AND TECHNOLOGY, v. 24, n. 4, p. 448-461, APR 3 2014. (10/06374-1, 11/17877-7)

MACHADO, SUSANE MOREIRA; PACHECO-SOARES, CRISTINA; MARCIANO, FERNANDA ROBERTA; LOBO, ANDERSON OLIVEIRA; DA SILVA, NEWTON SOARES. Photodynamic therapy in the cattle protozoan Tritrichomonas foetus cultivated on superhydrophilic carbon nanotube. Materials Science & Engineering C-Materials for Biological Applications, v. 36, p. 180-186, MAR 1 2014. (11/20345-7, 08/06654-4, 11/17877-7)

ZANIN, H.; CERAGIOLI, H. J.; PETERLEVITZ, A. C.; BARANAUSKAS, VITOR; MARCIANO, F. R.; LOBO, A. O.. Field emission properties of the graphenated carbon nanotube electrode. Applied Surface Science, v. 324, p. 174-178, JAN 1 2015. (11/20345-7, 14/02163-7, 11/17877-7)

RAMOS, B. C.; SAITO, E.; TRAVA-AIROLDI, V. J.; LOBO, A. O.; MARCIANO, F. R.. Diamond-like carbon electrochemical corrosion resistance by addition of nanocrystalline diamond particles for biomedical applications. SURFACE & COATINGS TECHNOLOGY, v. 259, n. C, p. 732-736, NOV 25 2014. (11/20345-7, 11/17877-7)

SAITO, E.; ANTUNES, E. F.; ZANIN, H.; MARCIANO, F. R.; LOBO, A. O.; TRAVA-AIROLDI, V. J.; CORAT, E. J.. Oxygen Plasma Exfoliated Vertically-Aligned Carbon Nanotubes as Electrodes for Ultrasensitive Stripping Detection of Pb2+. Journal of the Electrochemical Society, v. 161, n. 5, p. H321-H325, 2014. (11/20345-7, 11/17877-7)

RODRIGUES, BRUNO V. M.; LEITE, NELLY C. S.; CAVALCANTI, BRUNO DAS NEVES; DA SILVA, NEWTON S.; MARCIANO, FERNANDA R.; CORAT, EVALDO J.; WEBSTER, THOMAS J.; LOBO, ANDERSON O.. Graphene oxide/multi-walled carbon nanotubes as nanofeatured scaffolds for the assisted deposition of nanohydroxyapatite: characterization and biological evaluation. INTERNATIONAL JOURNAL OF NANOMEDICINE, v. 11, p. 2569-2585, 2016. (11/20345-7, 15/08523-8, 13/20054-8, 11/17877-7)

WACHESK, C. C.; TRAVA-AIROLDI, V. J.; DA-SILVA, N. S.; LOBO, A. O.; MARCIANO, F. R.. The Influence of Titanium Dioxide on Diamond-Like Carbon Biocompatibility for Dental Applications. Journal of Nanomaterials, 2016. (11/20345-7, 13/20054-8, 12/15857-1, 11/17877-7)

ZOGBI, JR., M. M.; SAITO, E.; ZANIN, H.; MARCIANO, F. R.; LOBO, A. O.. Hydrothermal-electrochemical synthesis of nano-hydroxyapatite crystals on superhydrophilic vertically aligned carbon nanotubes. Materials Letters, v. 132, p. 70-74, OCT 1 2014. (11/20345-7, 12/15773-2, 11/17877-7)

RODRIGUES, BRUNO V. M.; RAZZINO, CLAUDIA A.; OLIVEIRA, FRANCILIO DE CARVALHO; MARCIANO, FERNANDA R.; LOBO, ANDERSON O.. On the design and properties of scaffolds based on vertically aligned carbon nanotubes transferred onto electrospun poly (lactic acid) fibers. MATERIALS & DESIGN, v. 127, p. 183-192, AUG 5 2017. (15/08523-8, 11/17877-7, 15/09697-0, 16/00575-1)

RICCI, R.; LEITE, N. C. S.; DA-SILVA, N. S.; PACHECO-SOARES, C.; CANEVARI, R. A.; MARCIANO, F. R.; WEBSTER, T. J.; LOBO, A. O.. Graphene oxide nanoribbons as nanomaterial for bone regeneration: Effects on cytotoxicity, gene expression and bactericidal effect. Materials Science & Engineering C-Materials for Biological Applications, v. 78, p. 341-348, SEP 1 2017. (11/17877-7, 11/20345-7, 13/14464-9, 13/20054-8, 15/09697-0, 09/18440-1, 16/00575-1)

OLIVEIRA, C. A. G. S.; STEIN, M. F.; SAITO, E.; ZANIN, H.; VIEIRA, L. S.; RANIERO, L.; TRAVA-AIROLDI, V. J.; LOBO, A. O.; MARCIANO, F. R.. Effect of gold oxide incorporation on electrochemical corrosion resistance of diamond-like carbon. DIAMOND AND RELATED MATERIALS, v. 53, p. 40-44, MAR 2015. (11/20345-7, 14/02163-7, 11/17877-7)

DIAS, VANESSA; MACIEL, HOMERO; FRAGA, MARIANA; LOBO, ANDERSON O.; PESSOA, RODRIGO; MARCIANO, FERNANDA R.. Atomic Layer Deposited TiO2 and Al2O3 Thin Films as Coatings for Aluminum Food Packaging Application. MATERIALS, v. 12, n. 4, FEB 2 2019. (14/18139-8, 11/50773-0, 11/17877-7, 12/15857-1, 11/20345-7, 16/00575-1, 15/05956-0, 15/09697-0, 18/01265-1, 15/10876-6)

SOUZA, LUCAS; LOPES, JOAO HENRIQUE; ENCARNACAO, DAVI; MAZALI, ITALO ODONE; MARTIN, RICHARD ALAN; CAMILLI, JOSE ANGELO; BERTRAN, CELSO APARECIDO. Comprehensive in vitro and in vivo studies of novel melt-derived Nb-substituted 45S5 bioglass reveal its enhanced bioactive properties for bone healing. SCIENTIFIC REPORTS, v. 8, AUG 24 2018. (10/00863-0, 11/09240-9, 11/17877-7)

SILVA, ALINE DOS SANTOS; MANZOLLI RODRIGUES, BRUNO VINICIUS; OLIVEIRA, FRANCILIO CARVALHO; CARVALHO, JANCINEIDE OLIVEIRA; REIS DE VASCONCELLOS, LUANA MAROTTA; RIBEIRO DE ARAUJO, JULIANI CAROLINE; MARCIANO, FERNANDA ROBERTA; LOBO, ANDERSON OLIVEIRA. Characterization and in vitro and in vivo assessment of poly(butylene adipate-co-terephthalate)/nano-hydroxyapatite composites as scaffolds for bone tissue engineering. JOURNAL OF POLYMER RESEARCH, v. 26, n. 2, FEB 1 2019. (15/01259-3, 16/04618-7, 11/20345-7, 11/17877-7)

LOBO, ANDERSON O.; ZANIN, HUDSON; SIQUEIRA, IDALIA A. W. B.; LEITE, NELLY C. S.; MARCIANO, FERNANDA R.; CORAT, EVALDO J.. Effect of ultrasound irradiation on the production of nHAp/MWCNT nanocomposites. Materials Science & Engineering C-Materials for Biological Applications, v. 33, n. 7, p. 4305-4312, OCT 1 2013. (11/20345-7, 11/17877-7)

GRANATO, ALESSANDRO E. C.; RIBEIRO, ANDRE C.; MARCIANO, FERNANDA R.; RODRIGUES, BRUNO V. M.; LOBO, ANDERSON O.; PORCIONATTO, MARIMELIA. Polypyrrole increases branching and neurite extension by Neuro2A cells on PBAT ultrathin fibers. Nanomedicine-Nanotechnology Biology and Medicine, v. 14, n. 6, p. 1753-1763, AUG 2018. (11/20345-7, 12/00652-5, 11/17877-7)

STOCCO, THIAGO DOMINGUES; ANTONIOLI, ELIANE; VAZ ELIAS, CONCEICAO DE MARIA; MANZOLLI RODRIGUES, BRUNO VINICIUS; WALTRICK DE BRITO SIQUEIRA, IDALIA APARECIDA; FERRETTI, MARIO; MARCIANO, FERNANDA ROBERTA; LOBO, ANDERSON OLIVEIRA. Cell Viability of Porous Poly(d,l-lactic acid)/Vertically Aligned Carbon Nanotubes/Nanohydroxyapatite Scaffolds for Osteochondral Tissue Engineering. MATERIALS, v. 12, n. 6, MAR 2 2019. (11/17877-7)

SILVA, EDMUNDO; REIS DE VASCONCELLOS, LUANA MAROTTA; RODRIGUES, BRUNO V. M.; DOS SANTOS, DANILO MARTINS; CAMPANA-FILHO, SERGIO P.; MARCIANO, FERNANDA ROBERTA; WEBSTER, THOMAS J.; LOBO, ANDERSON OLIVEIRA. PDLLA honeycomb-like scaffolds with a high loading of superhydrophilic graphene/multi-walled carbon nanotubes promote osteoblast in vitro functions and guided in vivo bone regeneration. Materials Science & Engineering C-Materials for Biological Applications, v. 73, p. 31-39, APR 1 2017. (11/17877-7, 11/20345-7, 15/09697-0, 15/08523-8, 16/00575-1)

LOPES, JOAO H.; SOUZA, LUCAS P.; DOMINGUES, JULIANA A.; FERREIRA, V, FILIPE; HAUSEN, MOEMA DE ALENCAR; CAMILLI, JOSE A.; MARTIN, RICHARD A.; DE REZENDE DUEK, ELIANA A.; MAZALI, ITALO O.; BERTRAN, CELSO A.. In vitro and in vivo osteogenic potential of niobium-doped 45S5 bioactive glass: A comparative study. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, v. 108, n. 4, p. 1372-1387, MAY 2020. (11/17877-7, 11/09240-9, 10/05394-9, 10/00863-0)

LOPES, JOAO H.; SOUZA, LUCAS P.; DOMINGUES, JULIANA A.; FERREIRA, V, FILIPE; HAUSEN, MOEMA DE ALENCAR; CAMILLI, JOSE A.; MARTIN, RICHARD A.; DE REZENDE DUEK, ELIANA A.; MAZALI, ITALO O.; BERTRAN, CELSO A.. In vitro and in vivo osteogenic potential of niobium-doped 45S5 bioactive glass: A comparative study. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, OCT 2019. (10/05394-9, 11/17877-7, 10/00863-0, 11/09240-9)

RAMOS, B. C.; SAITO, E.; TRAVA-AIROLDI, V. J.; LOBO, A. O.; MARCIANO, F. R.. Diamond-like carbon electrochemical corrosion resistance by addition of nanocrystalline diamond particles for biomedical applications. SURFACE & COATINGS TECHNOLOGY, v. 259, p. 5-pg., 2014-11-25. (11/20345-7, 11/17877-7)

RODRIGUES, BRUNO V. M.; LEITE, NELLY C. S.; CAVALCANTI, BRUNO DAS NEVES; DA SILVA, NEWTON S.; MARCIANO, FERNANDA R.; CORAT, EVALDO J.; WEBSTER, THOMAS J.; LOBO, ANDERSON O.. Graphene oxide/multi-walled carbon nanotubes as nanofeatured scaffolds for the assisted deposition of nanohydroxyapatite: characterization and biological evaluation. INTERNATIONAL JOURNAL OF NANOMEDICINE, v. 11, p. 17-pg., 2016-01-01. (15/08523-8, 11/20345-7, 13/20054-8, 11/17877-7)

CABRAL, TAYNA S.; SGOBBI, LIVIA F.; DELEZUK, JORGE; PESSOA, RODRIGO S.; LOBO, ANDERSON O.; RODRIGUES, BRUNO V. M.. Glucose sensing via a green and low-cost platform from electrospun poly (vinyl alcohol)/graphene quantum dots fibers. MATERIALS TODAY-PROCEEDINGS, v. 14, p. 6-pg., 2019-01-01. (15/08523-8, 17/18826-3, 11/17877-7)

SATO, TABATA P.; RODRIGUES, BRUNO V. M.; MELLO, DAPHNE C. R.; MUNCHOW, ELISEU A.; RIBEIRO, JULIANA S.; MACHADO, JOAO PAULO B.; VASCONCELLOS, LUANA M. R.; LOBO, ANDERSON O.; BOTTINO, MARCO C.; BORGES, ALEXANDRE L. S.. The role of nanohydroxyapatite on the morphological, physical, and biological properties of chitosan nanofibers. CLINICAL ORAL INVESTIGATIONS, v. 25, n. 5, p. 9-pg., 2020-10-13. (11/20345-7, 11/17877-7)

RODRIGUES, BRUNO V. M.; RAZZINO, CLAUDIA A.; OLIVEIRA, FRANCILIO DE CARVALHO; MARCIANO, FERNANDA R.; LOBO, ANDERSON O.. On the design and properties of scaffolds based on vertically aligned carbon nanotubes transferred onto electrospun poly (lactic acid) fibers. MATERIALS & DESIGN, v. 127, p. 10-pg., 2017-08-05. (15/09697-0, 16/00575-1, 15/08523-8, 11/17877-7)

COSTA, R. P. C.; LIMA-OLIVEIRA, D. A.; MARCIANO, F. R.; LOBO, A. O.; CORAT, E. J.; TRAVA-AIROLDI, V. J.. Comparative study of the tribological behavior under hybrid lubrication of diamond-like carbon films with different adhesion interfaces. Applied Surface Science, v. 285, p. 4-pg., 2013-11-15. (11/20345-7, 11/17877-7)

LILIAN DE SIQUEIRA; FÁBIO ROBERTO PASSADOR; ANDERSON OLIVEIRA LOBO; ELIANDRA DE SOUSA TRICHÊS. Morphological, thermal and bioactivity evaluation of electrospun PCL/β-TCP fibers for tissue regeneration. POLIMEROS-CIENCIA E TECNOLOGIA, v. 29, n. 1, 2019-03-18. (10/00863-0, 11/17877-7)

PESSOA, R. S.; DOS SANTOS, V. P.; CARDOSO, S. B.; DORIA, A. C. O. C.; FIGUEIRA, F. R.; RODRIGUES, B. V. M.; TESTONI, G. E.; FRAGA, M. A.; MARCIANO, F. R.; LOBO, A. O.; et al. TiO2 coatings via atomic layer deposition on polyurethane and polydimethylsiloxane substrates: Properties and effects on C. albicans growth and inactivation process. Applied Surface Science, v. 422, p. 73-84, NOV 15 2017. (11/50773-0, 12/15857-1, 11/17877-7, 11/20345-7, 15/10876-6, 15/09697-0, 15/08523-8, 16/00575-1, 15/05956-0)

SALLES, GEISA NOGUEIRA; DOS SANTOS PEREIRA, FERNANDA APARECIDA; PACHECO-SOARES, CRISTINA; MARCIANO, FERNANDA ROBERTA; HOLSCHER, CHRISTIAN; WEBSTER, THOMAS J.; LOBO, ANDERSON OLIVEIRA. A Novel Bioresorbable Device as a Controlled Release System for Protecting Cells from Oxidative Stress from Alzheimer's Disease. Molecular Neurobiology, v. 54, n. 9, p. 6827-6838, NOV 2017. (11/17877-7, 11/20345-7, 15/09697-0, 14/20561-0, 16/00575-1)

METOKI, N.; ROSA, C. M. R.; ZANIN, H.; MARCIANO, F. R.; ELIAZ, N.; LOBO, A. O.. Electrodeposition and biomineralization of nano-beta-tricalcium phosphate on graphenated carbon nanotubes. SURFACE & COATINGS TECHNOLOGY, v. 297, p. 51-57, JUL 15 2016. (11/20345-7, 11/17877-7)

ZANIN, HUDSON; MANZOLLI RODRIGUES, BRUNO VINICIUS; RIBEIRO NETO, WILSON ALVES; SUMAN BRETAS, ROSARIO ELIDA; DA-SILVA, NEWTON SOARES; MARCIANO, FERNANDA ROBERTA; LOBO, ANDERSON OLIVEIRA. High loading of graphene oxide/multi-walled carbon nanotubes into PDLLA: A route towards the design of osteoconductive, bactericidal and non-immunogenic 3D porous scaffolds. Materials Chemistry and Physics, v. 177, p. 56-66, JUL 1 2016. (11/17877-7, 11/20345-7, 15/01259-3, 13/20054-8, 14/02163-7, 15/08523-8)

REIS DE VASCONCELLOS, LUANA MAROTTA; DO PRADO, RENATA FALCHETE; SARTORI, ELISA MATTIAS; SILVEIRA MENDONCA, DANIELA BACCELLI; MENDONCA, GUSTAVO; MARCIANO, FERNANDA ROBERTA; LOBO, ANDERSON OLIVEIRA. In vitro osteogenesis process induced by hybrid nanohydroxyapatite/graphene nanoribbons composites. JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, v. 30, n. 7, JUL 2019. (11/20345-7, 11/17877-7)

SILVA SOARES, LUIS EDUARDO; NAHORNY, SIDNEI; BRAGA, VIVIAN DE FARIA; MARCIANO, FERNANDA ROBERTA; BHATTACHARJEE, TANMOY T.; LOBO, ANDERSON OLIVEIRA. Raman spectroscopy-multivariate analysis related to morphological surface features on nanomaterials applied for dentin coverage. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, v. 228, MAR 5 2020. (11/20345-7, 11/17877-7, 16/00575-1, 13/11192-8, 14/21587-2, 15/09697-0, 14/02163-7)

RICCI, RITCHELLI; LOBO, ANDERSON OLIVEIRA; MARCIANO, FERNANDA ROBERTA. In Vitro Osteogenesis Stimulation via Nano-Hydroxyapatite/Carbon Nanotube Thin Films on Biomedical Stainless Steel. MATERIALS, v. 11, n. 9, SEP 2018. (11/20345-7, 18/12383-5, 11/17877-7)

ZAMPRONI, LAURA N.; GRINET, MARCO A. V. M.; MUNDIM, MAYARA T. V. V.; REIS, MARCELLA B. C.; GALINDO, LAYLA T.; MARCIANO, FERNANDA R.; LOBO, ANDERSON O.; PORCIONATTO, MARIMELIA. Rotary jet-spun porous microfibers as scaffolds for stem cells delivery to central nervous system injury. Nanomedicine-Nanotechnology Biology and Medicine, v. 15, n. 1, p. 98-107, JAN 2019. (12/00652-5, 09/05700-5, 11/20345-7, 11/17877-7)

SILVA SOARES, LUIS EDUARDO; MAGALHAES, JUAREZ DA SILVA; MARCIANO, FERNANDA ROBERTA; LOBO, ANDERSON OLIVEIRA. Surface characteristics of a modified acidulated phosphate fluoride gel with nano-hydroxyapatite coating applied on bovine enamel subjected to an erosive environment. MICROSCOPY RESEARCH AND TECHNIQUE, v. 81, n. 12, p. 1456-1466, DEC 2018. (11/17877-7, 11/20345-7, 13/14464-9, 07/07984-5, 09/18440-1)

STOCCO, T. D.; RODRIGUES, B. V. M.; MARCIANO, F. R.; LOBO, A. O.. Design of a novel electrospinning setup for the fabrication of biomimetic scaffolds for meniscus tissue engineering applications. Materials Letters, v. 196, p. 221-224, JUN 1 2017. (11/17877-7, 15/08523-8, 11/20345-7, 16/00575-1, 15/09697-0)

GRANATO, ALESSANDRO E. C.; RODRIGUES, BRUNO V. M.; RODRIGUES-JUNIOR, DORIVAL M.; MARCIANO, FERNANDA R.; LOBO, ANDERSON O.; PORCIONATTO, MARIMELIA A.. Magnetic super-hydrophilic carbon nanotubes/graphene oxide composite as nanocarriers of mesenchymal stem cells: Insights into the time and dose dependences. Materials Science & Engineering C-Materials for Biological Applications, v. 67, p. 694-701, OCT 1 2016. (11/20345-7, 12/00652-5, 15/08523-8, 11/17877-7)

COSTA, R. P. C.; LIMA-OLIVEIRA, D. A.; MARCIANO, F. R.; LOBO, A. O.; CORAT, E. J.; TRAVA-AIROLDI, V. J.. Comparative study of the tribological behavior under hybrid lubrication of diamond-like carbon films with different adhesion interfaces. Applied Surface Science, v. 285, n. B, p. 645-648, NOV 15 2013. (11/20345-7, 11/17877-7)

ALMEIDA, C. N.; RAMOS, B. C.; DA-SILVA, N. S.; PACHECO-SOARES, C.; TRAVA-AIROLDI, V. J.; LOBO, A. O.; MARCIANO, F. R.. Morphological analysis and cell viability on diamond-like carbon films containing nanocrystalline diamond particles. Applied Surface Science, v. 275, p. 258-263, JUN 15 2013. (11/20345-7, 11/17877-7)

GONCALVES, NATALIA I.; MUNCHOW, ELISEU A.; SANTOS, JESSICA D.; SATO, TABATA P.; DE OLIVEIRA, LETICIA R.; DE ARRUDA PAES-JUNIOR, TARCISIO J.; BOTTINO, MARCO C.; BORGES, ALEXANDRE L. S.. The role of polymeric nanofibers on the mechanical behavior of polymethyl methacrylate resin. JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS, v. 112, DEC 2020. (11/17877-7, 11/20345-7)

DE SIQUEIRA, LILIAN; PASSADOR, FABIO ROBERTO; LOBO, ANDERSON OLIVEIRA; TRICHES, ELIANDRA DE SOUSA. Morphological, thermal and bioactivity evaluation of electrospun PCL/beta-TCP fibers for tissue regeneration. POLIMEROS-CIENCIA E TECNOLOGIA, v. 29, n. 1, 2019. (11/17877-7, 10/00863-0)

IRINEU, JOAO A. F.; MARSI, TERESA C. O.; SANTOS, TIAGO G.; CORAT, EVALDO J.; MARCIANO, FERNANDA R.; LOBO, ANDERSON O.. Efficient method to produce biomineralizated nanohydroxyapatite/vertically aligned multiwalled carbon nanotube scaffolds. Materials Letters, v. 79, p. 166-169, JUL 15 2012. (11/17877-7)

WACHESK, C. C.; PIRES, C. A. F.; RAMOS, B. C.; TRAVA-AIROLDI, V. J.; LOBO, A. O.; PACHECO-SOARES, C.; MARCIANO, F. R.; DA-SILVA, N. S.. Cell viability and adhesion on diamond-like carbon films containing titanium dioxide nanoparticles. Applied Surface Science, v. 266, p. 176-181, FEB 1 2013. (11/20345-7, 11/17877-7)

BRAZIL, TAYRA RODRIGUES; DAS NEVES, MARCELE FLORENCIO; REGIANI, INACIO; MARCIANO, FERNANDA ROBERTA; LOBO, ANDERSON OLIVEIRA. Proposed model for biomineralization of novel nanohydroxyapatite/vertically aligned multiwalled carbon nanotube scaffolds. MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS, v. 16, n. 3, p. 661-667, MAY-JUN 2013. (11/20345-7, 12/02159-4, 11/17877-7)

SIQUEIRA, IDALIA A. W. B.; OLIVEIRA, CILIANA A. G. S.; ZANIN, HUDSON; GRINET, MARCO A. V. M.; GRANATO, ALESSANDRO E. C.; PORCIONATTO, MARIMELIA A.; MARCIANO, FERNANDA R.; LOBO, ANDERSON O.. Bioactivity behaviour of nano-hydroxyapatite/freestanding aligned carbon nanotube oxide composite. JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, v. 26, n. 2, FEB 2015. (11/20345-7, 14/02163-7, 13/07696-0, 11/17877-7)

ZANIN, H.; MAY, P. W.; LOBO, A. O.; SAITO, E.; MACHADO, J. P. B.; MARTINS, G.; TRAVA-AIROLDI, V. J.; CORAT, E. J.. Effect of Multi-Walled Carbon Nanotubes Incorporation on the Structure, Optical and Electrochemical Properties of Diamond-Like Carbon Thin Films. Journal of the Electrochemical Society, v. 161, n. 5, p. H290-H295, 2014. (11/17877-7)

ZANIN, HUDSON; SAITO, EDUARDO; MARCIANO, FERNANDA ROBERTA; CERAGIOLI, HELDER JOSE; CAMPOS GRANATO, ALESSANDRO EUSTAQUIO; PORCIONATTO, MARIMELIA; LOBO, ANDERSON OLIVEIRA. Fast preparation of nano-hydroxyapatite/superhydrophilic reduced graphene oxide composites for bioactive applications. JOURNAL OF MATERIALS CHEMISTRY B, v. 1, n. 38, p. 4947-4955, 2013. (11/20345-7, 11/17877-7)

LOPES, JOAO HENRIQUE; MAGALHAE, JESSICA APARECIDA; GOUUEIA, RUBIA FIGUEREDO; BERTRAN, CELSO APARECIDO; MOTISUKE, MARIANA; CAMARGO, SAMIRA E. A.; TRICHES, ELIANDRA DE SOUSA. Hierarchical structures of beta-TCP/45S5 bioglass hybrid scaffolds prepared by gelcasting. JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS, v. 62, p. 10-23, SEP 2016. (10/00863-0, 11/09240-9, 11/17877-7)

RODRIGUES, BRUNO V. M.; SILVA, ALINE S.; MELO, GABRIELA F. S.; VASCONSCELLOS, LUANA M. R.; MARCIANO, FERNANDA R.; LOBO, ANDERSON O.. Influence of low contents of superhydrophilic MWCNT on the properties and cell viability of electrospun poly (butylene adipate-co-terephthalate) fibers. Materials Science & Engineering C-Materials for Biological Applications, v. 59, p. 782-791, FEB 1 2016. (15/01259-3, 15/08523-8, 11/20345-7, 11/17877-7)

SANTANA-MELO, GABRIELA F.; RODRIGUES, BRUNO V. M.; DA SILVA, EDMUNDO; RICCI, RITCHELLI; MARCIANO, FERNANDA R.; WEBSTER, THOMAS J.; VASCONCELLOS, LUANA M. R.; LOBO, ANDERSON O.. Electrospun ultrathin PBAT/nHAp fibers influenced the in vitro and in vivo osteogenesis and improved the mechanical properties of neoformed bone. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 155, p. 544-552, JUL 1 2017. (11/17877-7, 11/20345-7, 15/09697-0, 15/08523-8, 16/00575-1)

NAHORNY, SIDNEI; ZANIN, HUDSON; CHRISTINO, VINIE ABREU; MARCIANO, FERNANDA ROBERTA; OLIVEIRA LOBO, ANDERSON; SILVA SOARES, LUIS EDUARDO. Multi-walled carbon nanotubes/graphene oxide hybrid and nanohydroxyapatite composite: A novel coating to prevent dentin erosion. Materials Science & Engineering C-Materials for Biological Applications, v. 79, p. 199-208, OCT 1 2017. (14/21587-2, 11/17877-7, 11/20345-7, 15/50093-0, 13/20825-4, 14/02163-7, 15/09697-0, 13/11192-8, 16/00575-1)

GRINET, MARCO A. V. M.; ZANIN, HUDSON; CAMPOS GRANATA, ALESSANDRO EUSTAQUIO; PORCIONATTO, MARIMELIA; MARCIANO, FERNANDA ROBERTA; LOBO, ANDERSON OLIVEIRA. Fast preparation of free-standing nanohydroxyapatite-vertically aligned carbon nanotube scaffolds. JOURNAL OF MATERIALS CHEMISTRY B, v. 2, n. 9, p. 1196-1204, MAR 7 2014. (11/20345-7, 13/07696-0, 11/17877-7)

SIQUEIRA, IDALIA A. W. B.; CORAT, MARCUS ALEXANDRE F.; CAVALCANTI, BRUNO DAS NEVES; RIBEIRO NETO, WILSON ALVES; MARTIN, AIRTON ABRAHAO; SUMAN BRETAS, ROSARIO ELIDA; MARCIANO, FERNANDA ROBERTA; LOBO, ANDERSON OLIVEIRA. In Vitro and in Vivo Studies of Novel Poly(D,L-lactic acid), Superhydrophilic Carbon Nanotubes, and Nanohydroxyapatite Scaffolds for Bone Regeneration. ACS APPLIED MATERIALS & INTERFACES, v. 7, n. 18, p. 9385-9398, MAY 13 2015. (11/20345-7, 12/02159-4, 11/17877-7)

ZANIN, H.; ROSA, C. M. R.; ELIAZ, N.; MAY, P. W.; MARCIANO, F. R.; LOBO, A. O.. Assisted deposition of nano-hydroxyapatite onto exfoliated carbon nanotube oxide scaffolds. NANOSCALE, v. 7, n. 22, p. 10218-10232, 2015. (11/20345-7, 14/02163-7, 11/17877-7)

ZANIN, H.; HOLLANDA, L. M.; CERAGIOLI, H. J.; FERREIRA, M. S.; MACHADO, D.; LANCELLOTTI, M.; CATHARINO, R. R.; BARANAUSKAS, V.; LOBO, A. O.. Carbon nanoparticles for gene transfection in eukaryotic cell lines. Materials Science & Engineering C-Materials for Biological Applications, v. 39, p. 359-370, JUN 1 2014. (11/17195-3, 11/17877-7)

MACHADO-PAULA, M. M.; CORAT, M. A. F.; LANCELLOTTI, M.; MI, G.; MARCIANO, F. R.; VEGA, M. L.; HIDALGO, A. A.; WEBSTER, T. J.; LOBO, A. O.. A comparison between electrospinning and rotary-jet spinning to produce PCL fibers with low bacteria colonization. Materials Science & Engineering C-Materials for Biological Applications, v. 111, JUN 2020. (11/20345-7, 15/08523-8, 11/17877-7, 14/50869-6, 14/16295-2, 15/09697-0)

ZANIN, H.; MARGRAF-FERREIRA, A.; DA SILVA, N. S.; MARCIANO, F. R.; CORAT, E. J.; LOBO, A. O.. Graphene and carbon nanotube composite enabling a new prospective treatment for trichomoniasis disease. Materials Science & Engineering C-Materials for Biological Applications, v. 41, p. 65-69, AUG 1 2014. (11/20345-7, 08/06654-4, 11/17877-7)

WACHESK, C. C.; TRAVA-AIROLDI, V. J.; DA-SILVA, N. S.; LOBO, A. O.; MARCIANO, F. R.. The Influence of Titanium Dioxide on Diamond-Like Carbon Biocompatibility for Dental Applications. Journal of Nanomaterials, v. 2016, p. 7-pg., 2016-01-01. (12/15857-1, 11/20345-7, 13/20054-8, 11/17877-7)

ANDRADE, P. O.; SANTO, A. M. E.; COSTA, M. M.; LOBO, A. O.; BEAUREPAIRE, E; PAVONE, FS; SO, PTC. Production of rotary jet spun ultrathin fibers of poly-butylene adipate-co- terephthalate (PBAT) filled with nanocomposites. ADVANCES IN MICROSCOPIC IMAGING, v. 10414, p. 7-pg., 2017-01-01. (11/20345-7, 13/20054-8, 11/17877-7)

SILVA, AMANDA ARAUJO; PINHEIRO, ROMARIO ARAUJO; RAZZINO, CLAUDIA DO AMARAL; TRAVA-AIROLDI, VLADIMIR JESUS; CORAT, EVALDO JOSE. Thin-film nanocomposites of BDD/CNT deposited on carbon fiber. DIAMOND AND RELATED MATERIALS, v. 75, p. 7-pg., 2017-05-01. (12/15857-1, 11/17877-7, 15/02960-7)