| Texto completo | |
| Autor(es): |
de Lima, Lucas F.
;
Barbosa, Priscilla P.
;
Simeoni, Camila L.
;
de O. de Paula, Rosemeire F.
;
Proenca-Modena, Jose Luiz
;
de Araujo, William R.
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ACS APPLIED MATERIALS & INTERFACES; v. 15, n. 50, p. 13-pg., 2023-12-08. |
| Resumo | |
Monkeypox virus (MPXV) infection was classified as a public health emergency of international concern by the World Health Organization (WHO) in 2022, being transmitted between humans by large respiratory droplets, in contact with skin lesions, fomites, and sexually. Currently, there are no available accessible and simple-to-use diagnostic tests that accurately detect MPXV antigens for decentralized and frequent testing. Here, we report an electrochemical biosensor to detect MPXV antigens in saliva and plasma samples within 15 min using accessible materials. The electrochemical system was manufactured onto a paper substrate engraved by a CO2 laser machine, modified with gold nanostructures (AuNS) and a monoclonal antibody, enabling sensitive detection of A29 viral protein. The diagnostic test is based on the use of electrochemical impedance spectroscopy (EIS) and can be run by a miniaturized potentiostat connected to a smartphone. The impedimetric biosensing method presented excellent analytical parameters, enabling the detection of A29 glycoprotein in the concentration ranging from 1 x 10(-14) to 1 x 10(-7) g mL(-1), with a limit of detection (LOD) of 3.0 x 10(-16) g mL(-1). Furthermore, it enabled the detection of MPXV antigens in the concentration ranging from 1 x 10(-1) to 1 x 10(4) PFU mL(-1), with an LOD of 7.8 x 10(-3) PFU mL(-1). Importantly, no cross-reactivity was observed when our device was tested in the presence of other poxvirus and nonpoxvirus strains, indicating the adequate selectivity of our nanobiosensor for MPXV detection. Collectively, the nanobiosensor presents high greenness metrics associated with the use of a reproducible and large-scale fabrication method, an accessible and sustainable paper substrate, and a low volume of sample (2.5 mu L), which could facilitate frequent testing of MPXV at point-of-care (POC). (AU) | |
| Processo FAPESP: | 22/03250-7 - Desenvolvimento de biossensores portáteis e acessíveis para diagnósticos clínicos de infecções bacterianas e virais |
| Beneficiário: | William Reis de Araujo |
| Modalidade de apoio: | Auxílio à Pesquisa - Projeto Inicial |
| Processo FAPESP: | 18/08782-1 - Sensores químicos miniaturizados e integrados: novas plataformas de fabricação para aplicações biológicas, clínicas e ambientais |
| Beneficiário: | Mauro Bertotti |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 16/00194-8 - Patogênese e neurovirulência de vírus emergentes no Brasil |
| Beneficiário: | José Luiz Proença Módena |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 22/10442-0 - Rede PAMPA para avaliação do impacto da degradação ambiental na dinâmica de circulação de vírus emergentes e reemergentes na região Amazônica |
| Beneficiário: | José Luiz Proença Módena |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |