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| Autor(es): Mostrar menos - |
Ferreira, Davi Azevedo
;
Araujo Medeiros, Anna Beatriz
;
Soares, Mariana Mendonca
;
Lima, Essia de Almeida
;
Santos Lima de Oliveira, Gabriela Carolina
;
da Silva Leite, Mateus Bernardo
;
Machado, Matheus Vieira
;
Ferreira Perez Villar, Jose Augusto
;
Barbosa, Leandro Augusto
;
Scavone, Cristoforo
;
Moura, Marcelo Tigre
;
Rodrigues-Mascarenhas, Sandra
Número total de Autores: 12
|
| Tipo de documento: | Artigo Científico |
| Fonte: | CELLS; v. 13, n. 18, p. 19-pg., 2024-09-01. |
| Resumo | |
Cardiotonic steroids are known to bind to Na+/K+-ATPase and regulate several biological processes, including the immune response. The synthetic cardiotonic steroid gamma-Benzylidene Digoxin 8 (BD-8) is emerging as a promising immunomodulatory molecule, although it has remained largely unexplored. Therefore, we tested the immunomodulatory potential of BD-8 both in vitro and in vivo. Hence, primary mouse macrophages were incubated with combinations of BD-8 and the pro-inflammatory fungal protein zymosan (ZYM). Nitric oxide (NO) production was determined by Griess reagent and cytokines production was assessed by enzyme-linked immunosorbent assay. Inducible nitric oxide synthase (iNOS), reactive oxygen species (ROS), p-nuclear factor kappa B p65 (NF-kappa B p65), p-extracellular signal-regulated kinase (p-ERK), and p-p38 were evaluated by flow cytometry. Macrophages exposed to BD-8 displayed reduced phagocytic activity, NO levels, and production of the proinflammatory cytokine IL-1 beta induced by ZYM. Furthermore, BD-8 diminished the expression of iNOS and phosphorylation of NF-kappa B p65, ERK, and p38. Additionally, BD-8 exhibited anti-inflammatory capacity in vivo in a carrageenan-induced mouse paw edema model. Taken together, these findings demonstrate the anti-inflammatory activity of BD-8 and further reinforce the potential of cardiotonic steroids and their derivatives as immunomodulatory molecules. (AU) | |
| Processo FAPESP: | 21/06009-6 - Neuroplasticidade induzida pela ativação da sinalização Klotho, Na, K-ATPase mediadores da neuroinflamação e o sistema glutamatérgico como estratégia para novos alvos terapêuticos para o envelhecimento e doenças neurodegenerativas |
| Beneficiário: | Cristoforo Scavone |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |