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Entree


Functional Characterization of ent-Copalyl Diphosphate Synthase and Kaurene Synthase Genes from Coffea arabica L

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Autor(es):
Ivamoto-Suzuki, Suzana Tiemi ; Celedon, Jose Miguel ; Yuen, Macaire M. S. ; Kitzberger, Ci ; Silva Domingues, Douglas ; Bohlmann, Jorg ; Protasio Pereira, Luiz Filipe
Número total de Autores: 7
Tipo de documento: Artigo Científico
Fonte: Journal of Agricultural and Food Chemistry; v. 71, n. 42, p. 11-pg., 2023-10-10.
Resumo

The biochemical profile of coffee beans translates directly into quality traits, nutraceutical and health promoting properties of the coffee beverage. Ent-kaurene is the ubiquitous precursor for gibberellin biosynthesis in plants, but it also serves as an intermediate in specialized (i.e., secondary) diterpenoid metabolism that leads to a diversity of more than 1,000 different metabolites. Nutraceutical effects on human health attributed to diterpenes include antioxidant, anticarcinogenic, and anti-inflammatory properties. Cafestol (CAF) and kahweol (KAH) are two diterpenes found exclusively in the Coffea genus. Our objective was to identify and functionally characterize genes involved in the central step of ent-kaurene production. We identified 17 putative terpene synthase genes in the transcriptome of Coffea arabica. Two ent-copalyl diphosphate synthase (CaCPS) and three kaurene synthase (CaKS) were selected and manually annotated. Transcript expression profiles of CaCPS1 and CaKS3 best matched the CAF and KAH metabolite profiles in different tissues. CaCPS1 and CaKS3 proteins were heterologously expressed and functionally characterized. CaCPS1 catalyzes the cyclization of geranylgeranyl diphosphate (GGPP) to ent-copalyl diphosphate (ent-CPP), which is converted to ent-kaurene by CaKS3. Knowledge about the central steps of diterpene formation in coffee provides a foundation for future characterization of the subsequent enzymes involved in CAF and KAH biosynthesis. (AU)

Processo FAPESP: 17/01455-2 - Bases bioquímicas e moleculares da rede metabólica de diterpenos, compostos bioativos e naturais de cafeeiros
Beneficiário:Suzana Tiemi Ivamoto
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 16/10896-0 - A sinalização do ácido hexanóico pode modular o transcriptoma, metaboloma e o desenvolvimento de patógenos no cafeeiro?
Beneficiário:Douglas Silva Domingues
Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores