| Texto completo | |
| Autor(es): |
Fragelli, Bruna D. L.
;
Assis, Marcelo
;
Rodolpho, Joice M. A.
;
Godoy, Krissia F.
;
Libero, Laura O.
;
Anibal, Fernanda F.
;
Longo, Elson
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 257, p. 11-pg., 2024-06-07. |
| Resumo | |
The cytotoxic of alpha-Ag 2 WO 4 synthesized in different morphologies (cuboidal (AW-C), hexagonal rod -like (AWHRL) and nanometric rod -like (AW-NRL) was analyzed to understand the impact of morphological modulation on the toxicity of 3 T3 cell lines in the dark and when photoactivated by visible light. Pathways of toxicity were examined, such as parameters and electrostatic interaction, uptake, ion release and ROS production. Cytotoxicity was observed for all samples after reaching concentrations exceeding 7.8 mu g/mL. Uptake tests demonstrated that the samples were not internalized by cells, likely due to their negative surface charge. AW-NRL exhibited autophagy in the absence of light and during photoactivation, primarily attributed to its ability to generate singlet oxygen. Analyzing intercellular ROS and RNS production, AW-HRL induced an increase in NO through exposure to photo -generated hydroxyl radicals, while AW-NRL showed increases only at non-photoactivated concentrations and AW-C did not exhibit increases. Interestingly, in the dark, these cells showed a low propensity for apoptosis, with late apoptosis and necrosis being more pronounced. When photoactivated, this behavior changed, revealing predominantly apoptotic and late apoptotic cell death. There is a need for an understanding of how morphology can alter the biological properties of alpha-Ag 2 WO 4 to predict and optimize its effects on cellular responses. (AU) | |
| Processo FAPESP: | 13/07296-2 - CDMF - Centro de Desenvolvimento de Materiais Funcionais |
| Beneficiário: | Elson Longo da Silva |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |
| Processo FAPESP: | 22/06210-6 - Efeito antitumoral de diferentes morfologias de semicondutores a base de Ag contra células tumorais de bexiga |
| Beneficiário: | Bruna Dias de Lima Fragelli |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |