Glutamatergic and purinergic transmitters and astrocyte modulation in the synaptic transmission in the NTS of rats exposed to short-term sustained hypoxia

Autor(es)

Bazilio, Darlan S. ; Moraes, Davi J. A. ; Machado, Benedito H.

• Tipo de documento: Artigo Científico
• Fonte: AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY; v. 327, n. 4, p. 19-pg., 2024-10-05.

Resumo

There is evidence that astrocytes modulate synaptic transmission in the nucleus tractus solitarius (NTS) interacting with glutamatergic and purinergic mechanisms. Here, using in situ working heart-brainstem preparations, we evaluated the involvement of astrocyte and glutamatergic/purinergic neurotransmission in the processing of autonomic and respiratory pathways in the NTS of control and rats exposed to sustained hypoxia (SH). Baseline autonomic and respiratory activities and the responses to chemoreflex activation (KCN) were evaluated before and after microinjections of fluorocitrate (FCt, an astrocyte metabolic inhibitor), kynurenic acid, and pyridoxalphosphate-6-azophenyl-2 ',4 '-disulfonate (PPADS) (nonselective antagonists of glutamatergic and purinergic receptors) into the rostral aspect of the caudal commissural NTS. FCt had no effects on the baseline parameters evaluated but reduced the bradycardic response to chemoreflex activation in SH rats. FCt combined with kynurenic acid and PPADS in control rats reduced the baseline duration of expiration, which was attenuated after SH. FCt produced a large increase in PN frequency discharge in control rats, which was reduced after SH, indicating a reduction in the astrocyte modulation after SH. The data show that 1) the bradycardic component of the peripheral chemoreflex is reduced in SH rats after astrocytes inhibition, 2) the inhibition of astrocytes in the presence of double antagonists in the NTS affects the modulation of baseline duration of expiration in control but not in SH rats, and 3) the autonomic and respiratory responses to chemoreflex activation are mediated by glutamatergic and purinergic receptors in the rostral aspect of the caudal commissural NTS. (AU)

• Processo FAPESP: 18/15957-2 - Modulação astrocítica dos neurônios bulbares envolvidos com a geração e controle das atividades simpática e respiratória de roedores submetidos à hipóxia
• Beneficiário: Benedito Honorio Machado
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 19/21328-0 - Participação dos astrócitos na modulação dos neurônios do NTS envolvidos no processamento dos componentes cardiovascular e respiratório do quimiorreflexo de ratos submetidos à hipóxia mantida
• Beneficiário: Darlan da Silva Bazilio
• Modalidade de apoio: Bolsas no Brasil - Doutorado