| Texto completo | |
| Autor(es): |
Assugeni, Isabela Oliveira Sandrini
;
Bazon, Murilo Luiz
;
Pinto, Lucas Machado
;
Mainente, Leonardo Antonio Benedito
;
Brochetto-Braga, Marcia Regina
;
Zollner, Ricardo de Lima
;
Fernandes, Luis Gustavo Romani
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF IMMUNOLOGICAL METHODS; v. 522, p. 8-pg., 2023-09-15. |
| Resumo | |
Polybia paulista is a neotropical social wasp related to severe accidents and allergic reactions cases, including anaphylaxis, in southeastern Brazil. Antigen 5 (Poly p 5) is a major allergenic protein from its venom with potential use for component-resolved diagnostic. Therefore, the previous characterization of the immune response profile triggered by Poly p 5 should be evaluated. Recombinant Poly p 5 (rPoly p 5) was used to sensitize BALB/c mice with six weekly intradermal doses, and the specific antibody production and the functional profile of CD4+ T cells were assessed. rPoly p 5 induced the production of specific immunoglobulins (sIg) sIgE, sIgG1 and sIgG2a, which could recognize natural Poly p 5 presented in the venom of four different wasp species. rPoly p 5 stimulated in vitro the CD4+ T cells from immunized mice, which showed a significant proliferative response. These antigen-specific CD4+T cells produced IFN-gamma and IL-17A cytokines and increased ROR gamma T transcription factor expression. No differences between the control group and sensitized mice were found in IL-4 production and GATA-3 and T-bet expression. Interestingly, increased CD25+FoxP3+ regulatory T cells (Tregs) frequency was observed in the splenocyte cell cultures from rPoly p 5 immunized mice after the in vitro stimulation with both P. paulista venom extract and rPoly p 5. Here we showed that rPoly p 5 induces antigen specific antibodies capable of recognizing Antigen 5 in the venom of four wasp species and modulates antigen specific CD4+ T cells to IFN-gamma production response associated with a Th17 profile in sensitized mice. These findings emphasize the potential use of rPoly p 5 as an essential source of a major wasp allergen with significant immunological properties. (AU) | |
| Processo FAPESP: | 17/18422-0 - Avaliação dos efeitos do Antígeno 5 do veneno de Polybia paulista (Hymenoptera,Vespidae) na forma recombinante sobre a produção de mediadores inflamatórios e imunológicos in vitro para o desenvolvimento de kit diagnóstico |
| Beneficiário: | Márcia Regina Brochetto Braga |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 19/22024-5 - Modulação da produção de anticorpos aos componentes antigênicos do veneno de Polybia paulista e ativação de mastócitos em modelo murino experimental de alergia: efeitos da supressão bystander induzida por tolerância oral a antígeno proteico não correlato |
| Beneficiário: | Leonardo Antonio Benedito Mainente |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 17/07988-2 - Avaliação biológica e inflamatória dos efeitos do antígeno 5 do veneno de Polybia paulista (Hymenoptera, Vespidae) na forma recombinante, sobre células de linhagem macrofágica |
| Beneficiário: | Murilo Luiz Bazon |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 19/00729-7 - Modulação da resposta imunológica aos componentes antigênicos do veneno de Polybia paulista (Hymenoptera, Vepidae) em modelo experimental murino de alergia: efeitos da supressão bystander induzida por tolerância oral a antígeno proteico. |
| Beneficiário: | Luis Gustavo Romani Fernandes |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |