Upconverting Nanoparticle-based Enhanced Luminescence Lateral-Flow Assay for Urinary Biomarker Monitoring

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Arai, Marylyn Setsuko ; Kim, Hyunho ; Pascavis, Madeleine ; Cha, Baekdong ; Brambilla, Gabriel ; Cho, Young Kwan ; Park, Jinho ; Vilela, Raquel R. C. ; de Camargo, Andrea S. S. ; Castro, Cesar M. ; Lee, Hakho

• Tipo de documento: Artigo Científico
• Fonte: ACS APPLIED MATERIALS & INTERFACES; v. 16, n. 29, p. 9-pg., 2024-07-09.

Resumo

Development of efficient portable sensors for accurately detecting biomarkers is crucial for early disease diagnosis, yet remains a significant challenge. To address this need, we introduce the enhanced luminescence lateral-flow assay, which leverages highly luminescent upconverting nanoparticles (UCNPs) alongside a portable reader and a smartphone app. The sensor's efficiency and versatility were shown for kidney health monitoring as a proof of concept. We engineered Er3+- and Tm3+-doped UCNPs coated with multiple layers, including an undoped inert matrix shell, a mesoporous silica shell, and an outer layer of gold (UCNP@mSiO(2)@Au). These coatings synergistically enhance emission by over 40-fold and facilitate biomolecule conjugation, rendering UCNP@mSiO(2)@Au easy to use and suitable for a broad range of bioapplications. Employing these optimized nanoparticles in lateral-flow assays, we successfully detected two acute kidney injury-related biomarkers & horbar;kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL)& horbar;in urine samples. Using our sensor platform, KIM-1 and NGAL can be accurately detected and quantified within the range of 0.1 to 20 ng/mL, boasting impressively low limits of detection at 0.28 and 0.23 ng/mL, respectively. Validating our approach, we analyzed clinical urine samples, achieving biomarker concentrations that closely correlated with results obtained via ELISA. Importantly, our system enables biomarker quantification in less than 15 min, underscoring the performance of our novel UCNP-based approach and its potential as reliable, rapid, and user-friendly diagnostics. (AU)

• Processo FAPESP: 13/07793-6 - CEPIV - Centro de Ensino, Pesquisa e Inovação em Vidros
• Beneficiário: Edgar Dutra Zanotto
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 21/01170-3 - Nanossistemas luminescentes multifuncionais vítreos e cerâmicos para aplicações em termometria e oximetria
• Beneficiário: Pedro Felipe Garcia Martins da Costa
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 19/12588-9 - Biossensores ópticos para detecção rápida de bactérias patogênicas
• Beneficiário: Marylyn Setsuko Arai
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 21/11531-3 - Sensores portáteis baseados em nanomateriais para detecção de doenças infecciosas e câncer
• Beneficiário: Marylyn Setsuko Arai
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado